*Optimal dilutions/concentrations should be determined by the researcher.
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).
Not tested in other applications.
This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family.
10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant intracellular domain of human HER-2 protein
Protein A/G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
CD340 , ERBB2 , HER2 , HER2/neu , MLN 19 , NEU , NGL , TKR1 , erbb2 receptor tyrosine kinase 2 , Her2 , ErbB2 , Neu , EGFR2 , erb-b2 receptor tyrosine kinase 2
Cell membrane,Cytoplasm,Perinuclear region,Nucleus
This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]