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Her2 / ErbB2 antibody [ERB2/776]

Anti-Her2 / ErbB2 antibody [ERB2/776] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34781
Anti-Her2 / ErbB2 antibody [ERB2/776] used in Flow cytometry (FACS). GTX34781

Cat No. GTX34781

Host

Mouse

Clonality

Monoclonal

Clone Name

ERB2/776

Isotype

IgG1

Application

ICC/IF, FACS, ELISA

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1-2μg/ml
FACS 1-2μg/10⁶ cells
ELISA Assay dependent

Note :

ELISA
For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

138 kDa. ( Note )

Specificity/Sensitivity

This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant intracellular domain of human HER-2 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CD340 , ERBB2 , HER2 , HER2/neu , MLN 19 , NEU , NGL , TKR1 , erbb2 receptor tyrosine kinase 2 , Her2 , ErbB2 , Neu , EGFR2 , erb-b2 receptor tyrosine kinase 2

Cellular Localization

Cell membrane,Cytoplasm,Perinuclear region,Nucleus

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Her2 / ErbB2 antibody [ERB2/776] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34781

GTX34781 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34781 Her2 / ErbB2 antibody [ERB2/776].
Green : Primary antibody
Red : nucleus

Anti-Her2 / ErbB2 antibody [ERB2/776] used in Flow cytometry (FACS). GTX34781

GTX34781 FACS Image

FACS analysis of trypsinized MCF-7 cells using GTX34781 Her2 / ErbB2 antibody [ERB2/776].
Blue : Primary antibody
Red : Isotype control

REFERENCE

There are currently no references for Her2 / ErbB2 antibody [ERB2/776] (GTX34781). Be the first to share your publications with this product.

REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf