Her2 / ErbB2 antibody [ERB2/776]

Cat. No. GTX34781

Host	Mouse
Clonality	Monoclonal
Clone Name	ERB2/776
Isotype	IgG1
Application	ICC/IF, FACS, ELISA
Reactivity	Human
Package	100 μg ($529)

See all Her2 / ErbB2 products

Datasheet
Document

View product citations for antibody GTX34781 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ICC/IF	1-2μg/ml
FACS	1-2μg/10⁶ cells
ELISA	Assay dependent
Note : ELISA For ELISA coating, recommend using BSA-free format (please contact us for PBS only format).

Not tested in other applications.

Calculated MW

138 kDa. ( Note )

Product Note

This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant intracellular domain of human HER-2 protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CD340 , ERBB2 , HER2 , HER2/neu , MLN 19 , NEU , NGL , TKR1 , erbb2 receptor tyrosine kinase 2 , Her2 , ErbB2 , Neu , EGFR2 , erb-b2 receptor tyrosine kinase 2

Cellular Localization

Cell membrane,Cytoplasm,Perinuclear region,Nucleus

Background

This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]

Database

Gene ID: 2064 ERBB2
UniProt: P04626 ERBB2

Research Area

DATA IMAGES

GTX34781 FACS Image

FACS analysis of trypsinized MCF-7 cells using GTX34781 Her2 / ErbB2 antibody [ERB2/776].
Blue : Primary antibody
Red : Isotype control

GTX34781 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX34781 Her2 / ErbB2 antibody [ERB2/776].
Green : Primary antibody
Red : nucleus

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty