United States (US)

Her2 / ErbB2 antibody [ERB2/776]

Anti-Her2 / ErbB2 antibody [ERB2/776] used in IHC (Paraffin sections) (IHC-P). GTX34781

Cat No. GTX34781

Host Mouse
Clonality Monoclonal
Clone Name ERB2/776
Isotype IgG1
Application ICC/IF, IHC-P
Reactivity Human

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

Boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

138 kDa. ( Note )


This MAb is specific to c-erbB-2/HER-2 and shows minimal cross-reaction with other members of the family. C-erbB-2/HER-2 is a member of the EGFR family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.




Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.


Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species



Recombinant intracellular domain of human HER-2 protein


Ab purified from Bioreactor Concentrate by Protein A/G




For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


Erb-B2 Receptor Tyrosine Kinase 2,Cd340,Her-2,Her-2/Neu,Her2,Mln 19,Neu,Ngl,Tkr1,Erbb2


This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008]


Research Area

Anti-Her2 / ErbB2 antibody [ERB2/776] used in IHC (Paraffin sections) (IHC-P). GTX34781

GTX34781 IHC-P Image

Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with HER-2 Monoclonal Antibody (ERB2/776).

Package List Price ($)
$ 329