Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] (GTX111276) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] (GTX111276) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her4 / ErbB4 antibody [C1C3] (GTX111276) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

ERBB4 antibody [C1C3] detects ERBB4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.
Green: ERBB4 protein stained by ERBB4 antibody [C1C3] (GTX111276) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunoprecipitation of Her4 / ErbB4 protein from MCF-7 whole cell extract using 4.5 μg of Her4 / ErbB4 antibody [C1C3] (GTX111276).
Western blot analysis was performed using Her4 / ErbB4 antibody [C1C3] (GTX111276) and diluted at 1:500.
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using ERBB4(v-erb-A)(GTX111276) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min