GeneTex
United States (US)

Heregulin beta-1 recombinant protein

Heregulin-beta-1-recombinant-protein_WB_GTX65114-1_18121411_627.jpg

Cat No. GTX65114

Application WB, ELISA, Functional Assay, Apuri, Blocking
Reactivity Human
Species Human
APPLICATION

Application Note

The ED50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is < 0.5 ng/ml, corresponding to a specific activity of > 2x10^6 units/mg.

Calculated MW

7.5 kDa. ( Note )
PROPERTIES

Form

Lyophilized powder

Storage

Reconstitute in water to a concentration of 0.1-1.0 μg/μl. The solution can then be diluted into other aqueous buffers and stored at 4ºC for 1 week or –20ºC for future use.

Antigen Species

Human

Expression System

E. coli

Purification


Purity was assessed by SDS-PAGE (≥98%) and by HPLC (≥98%).

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Neu differentiation factor, SMDF, Neuregulin1 (NRG1-beta1), Neuregulin1 (NRG1beta1), Neuregulin1 (NRG1 beta1)

Background

Neuregulin/Heregulin is a family of structurally related polypeptide growth factors derived from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). To date, there are over 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extraCellular domain of the transmembrane NRG1 isoforms release soluble growth factors. HRG1-beta1 contains an Ig domain and an EGF-like domain that is necessary for direct binding to receptor tyrosine kinases erb3 and erb4. This binding induces erb3 and erb4 heterodimerization with erb2, stimulating intrinsic kinase activity, which leads to tyrosine phosphorylation. Although HRG1-beta1 biological effects is still unclear, it has been found to promote motility and invasiveness of breast cancer cells which may also involve up-regulation of expression and function of the autocrine motility-promoting factor (AMF). Recombinant human Heregulin-beta1 (HRG1-beta1) is a 7.5 kDa polypeptide consisting of only the EGF domain of heregulin-beta1 (65 amino acid residues).

Research Area

DATA IMAGES
Heregulin-beta-1-recombinant-protein_WB_GTX65114-1_18121411_627.jpg

GTX65114 WB Image

SDS-PAGE (15%) of purified HRG1-β protein:
Lane 1: Marker
Lane 2: HRG1-β protein (257-382 aa) -- 20 µg

Package List Price ($)
$ 259