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Herpes virus 6 gQ1 antibody [119]

Orthogonal
Anti-Herpes virus 6 gQ1 antibody [119] used in Flow cytometry (FACS). GTX00685
Anti-Herpes virus 6 gQ1 antibody [119] used in Western Blot (WB). GTX00685
Anti-Herpes virus 6 gQ1 antibody [119] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00685
Anti-Herpes virus 6 gQ1 antibody [119] used in Immunoprecipitation (IP). GTX00685

Cat. No. GTX00685

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 119

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, IP, ELISA

Reactivity

 Human herpesvirus 6
Package
50 μg ($399)
See all Herpes virus 6 gQ1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:3200
FACS 1:100
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Product Note

This antibody reacts with gQ1 of HHV6A and HHV6B. However, it can only be used for IP of HHV-6A but not recommended for IP of HHV-6B due to conformation specificity.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human herpesvirus 6

Immunogen

His6-tagged recombinant gQ1 of HHV-6A encoding 3-422 amino acids expressed in E. coli

Purification

Purified IgG
From tissue culture supernatant

Purity

90~95% by SDS-PAGE

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Human herpesvirus 6 Glycoprotein Q1 , Human herpesvirus 6 gQ1 , HHV-6 Glycoprotein Q1 , HHV-6 gQ1

Research Area

DATA IMAGES

Anti-Herpes virus 6 gQ1 antibody [119] used in Flow cytometry (FACS). GTX00685

GTX00685 FACS Image

FACS analysis of 293T cells overexpressing HHV-6A gQ1 protein using GTX00685 Herpes Virus 6 gQ1 antibody [119].
Black line : Control
Red line : Primary antibody
Fixation : 4% PFA
Permeabilization : 0.1% Triton-X100

Anti-Herpes virus 6 gQ1 antibody [119] used in Western Blot (WB). GTX00685

GTX00685 WB Image

Orthogonal

WB analysis of HSB-2 cells infected HHV-6A (M.O.I = 0.1 for 72 hrs) using GTX00685 Herpes Virus 6 gQ1 antibody [119]. HHV-6A gQ1 protein is detected as two glycoproteins with 80 kDa and 74 kDa molecular masses.
(-) : Non-treated
(H) : Treated with end-glycosilase H
(F) : Treated with peptide N-glycosidase F

Anti-Herpes virus 6 gQ1 antibody [119] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00685

GTX00685 ICC/IF Image

ICC/IF analysis of HSB-2 cells infected with HHV6A using GTX00685 Herpes Virus 6 gQ1 antibody [119].
Fixation : Cold Acetone

Anti-Herpes virus 6 gQ1 antibody [119] used in Immunoprecipitation (IP). GTX00685

GTX00685 IP Image

IP analysis of HSB-2 cell mock (lane 1) or infected with HHV-6A GS strain (lane 2) using GTX00685 Herpes Virus 6 gQ1 antibody [119].

REFERENCE

Clone Reference

Tang H et al. J Virol. 2011; 85 (21):11121-30 Human herpesvirus 6 glycoprotein complex formation is required for folding and trafficking of the gH/gL/gQ1/gQ2 complex and its cellular receptor binding.
Application : WB, FACS, IP
Akkapaiboon P et al. J Virol. 2004; 78 (15):7969-83 Intracellular processing of human herpesvirus 6 glycoproteins Q1 and Q2 into tetrameric complexes expressed on the viral envelope.
Application : WB, ICC/IF, IP
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399