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Herpes Virus 6 gQ1 antibody [119]

Anti-Herpes Virus 6 gQ1 antibody [119] used in Immunoprecipitation (IP). GTX00685
Anti-Herpes Virus 6 gQ1 antibody [119] used in Flow cytometry (FACS). GTX00685
Anti-Herpes Virus 6 gQ1 antibody [119] used in Western Blot (WB). GTX00685
Anti-Herpes Virus 6 gQ1 antibody [119] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00685

Cat No. GTX00685

Host

Mouse

Clonality

Monoclonal

Clone Name

119

Isotype

IgG1

Application

WB, ICC/IF, FACS, IP, ELISA

Reactivity

Human herpesvirus 6
Package
50 μg ($359)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:3200
FACS 1:100
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Specificity/Sensitivity

This antibody reacts with gQ1 of HHV6A and HHV6B. However, it can only be used for IP of HHV-6A but not recommended for IP of HHV-6B due to conformation specificity.

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human herpesvirus 6

Immunogen

His6-tagged recombinant gQ1 of HHV-6A encoding 3-422 amino acids expressed in E. coli

Purification

Purified IgG
From tissue culture supernatant

Purity

90~95% by SDS-PAGE

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

DATA IMAGES

Anti-Herpes Virus 6 gQ1 antibody [119] used in Immunoprecipitation (IP). GTX00685

GTX00685 IP Image

IP analysis of HSB-2 cell mock (lane 1) or infected with HHV-6A GS strain (lane 2) using GTX00685 Herpes Virus 6 gQ1 antibody [119].

Anti-Herpes Virus 6 gQ1 antibody [119] used in Flow cytometry (FACS). GTX00685

GTX00685 FACS Image

FACS analysis of 293T cells overexpressing HHV-6A gQ1 protein using GTX00685 Herpes Virus 6 gQ1 antibody [119].
Black line : Control
Red line : Primary antibody
Fixation : 4% PFA
Permeabilization : 0.1% Triton-X100

Anti-Herpes Virus 6 gQ1 antibody [119] used in Western Blot (WB). GTX00685

GTX00685 WB Image

WB analysis of HSB-2 cells infected HHV-6A (M.O.I = 0.1 for 72 hrs) using GTX00685 Herpes Virus 6 gQ1 antibody [119]. HHV-6A gQ1 protein is detected as two glycoproteins with 80 kDa and 74 kDa molecular masses.
(-) : Non-treated
(H) : Treated with end-glycosilase H
(F) : Treated with peptide N-glycosidase F

Anti-Herpes Virus 6 gQ1 antibody [119] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00685

GTX00685 ICC/IF Image

ICC/IF analysis of HSB-2 cells infected with HHV6A using GTX00685 Herpes Virus 6 gQ1 antibody [119].
Fixation : Cold Acetone

REFERENCE

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Package List Price ($)
$ 359