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Histone H1.0 antibody

Orthogonal
Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462
Anti-Histone H1.0 antibody used in Western Blot (WB). GTX114462
Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462
Anti-Histone H1.0 antibody used in Western Blot (WB). GTX114462
Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462
Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462
Anti-Histone H1.0 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114462
Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462
Anti-Histone H1.0 antibody used in Western Blot (WB). GTX114462
Anti-Histone H1.0 antibody used in Western Blot (WB). GTX114462
Anti-Histone H1.0 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114462

Cat. No. GTX114462

Reference 8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Histone H1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

mouse liver , HepG2 nuclear extract

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Histone H1.0. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173658

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

H1 histone family member 0 , H10 , H1FV

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462

GTX114462 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Histone H1.0(GTX114462) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H1.0 antibody used in Western Blot (WB). GTX114462

GTX114462 WB Image

Histone H1 antibody detects Histone H1 protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (GTX114462) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462

GTX114462 IHC-P Image

Histone H1.0 antibody detects Histone H1.0 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H1.0 antibody (GTX114462) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H1.0 antibody used in Western Blot (WB). GTX114462

GTX114462 WB Image

Orthogonal

Histone H1 antibody detects Histone H1 protein by western blot analysis. HepG2 whole cell extracts and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (GTX114462) at a dilution of 1:10000 and developed with Trident femto Western HRP Substrate (GTX14698). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462

GTX114462 IHC-P Image

Histone H1.0 antibody detects Histone H1.0 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H1.0 antibody (GTX114462) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462

GTX114462 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Histone H1.0(GTX114462) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H1.0 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114462

GTX114462 ICC/IF Image

Histone H1.0 antibody detects Histone H1.0 protein at nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H1.0 stained by Histone H1.0 antibody (GTX114462) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462

GTX114462 IHC-P Image

Histone H1.0 antibody detects Histone H1.0 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H1.0 antibody (GTX114462) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H1.0 antibody used in Western Blot (WB). GTX114462

GTX114462 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1.0 antibody (GTX114462) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H1.0 antibody used in Western Blot (WB). GTX114462

GTX114462 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1.0 antibody (GTX114462) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Histone H1.0 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114462

GTX114462 ICC/IF Image

Histone H1.0 antibody detects Histone H1.0 protein at nucleus by immunofluorescent analysis.
Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H1.0 stained by Histone H1.0 antibody (GTX114462) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

REFERENCE

Application Reference

Ide K et al. J Biol Chem 2021; 297 (1) : 100819 The dipeptide prolyl-hydroxyproline promotes cellular homeostasis and lamellipodia-driven motility via active β1-integrin in adult tendon cells.
Reactivity : Human
Park YJ et al. Oxid Med Cell Longev 2021; 2021 : 6683033 PAR2 Deficiency Induces Mitochondrial ROS Generation and Dysfunctions, Leading to the Inhibition of Adipocyte Differentiation.
Pinto DO et al. Retrovirology 2021; 18 (1) : 6 Extracellular vesicles from HTLV-1 infected cells modulate target cells and viral spread.
Application : WB
Reactivity : Human
Kuo MW et al. J Biomed Sci 2021; 28 (1) : 7 Yulink, predicted from evolutionary analysis, is involved in cardiac function.
Application : WB
Reactivity : Mouse
Shimamoto Y et al. Mol Biol Cell 2017; 28 (11) : 1580-1589 Nucleosome-nucleosome interactions via histone tails and linker DNA regulate nuclear rigidity.
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169