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Histone H1.0 antibody

Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462
Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462
Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462
Anti-Histone H1.0 antibody used in Western Blot (WB). GTX114462
Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462
Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462
Anti-Histone H1.0 antibody used in Western Blot (WB). GTX114462

Cat No. GTX114462

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:20000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

HepG2 , HepG2 nuclear extract , mouse liver
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Histone H1.0. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

H1 histone family member 0,H10,H1FV

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq]

Database

DATA IMAGES
Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462

GTX114462 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Histone H1.0(GTX114462) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462

GTX114462 IHC-P Image

Histone H1.0 antibody detects Histone H1.0 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H1.0 antibody (GTX114462) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462

GTX114462 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SkHep1 xenograft, using Histone H1.0(GTX114462) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H1.0 antibody used in Western Blot (WB). GTX114462

GTX114462 WB Image

Histone H1 antibody detects Histone H1 protein by western blot analysis. HepG2 whole cell extracts and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (GTX114462) at a dilution of 1:10000 and developed with Trident femto Western HRP Substrate (GTX14698). The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462

GTX114462 IHC-P Image

Histone H1.0 antibody detects Histone H1.0 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H1.0 antibody (GTX114462) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H1.0 antibody used in IHC (Paraffin sections) (IHC-P). GTX114462

GTX114462 IHC-P Image

Histone H1.0 antibody detects Histone H1.0 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H1.0 antibody (GTX114462) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H1.0 antibody used in Western Blot (WB). GTX114462

GTX114462 WB Image

Histone H1 antibody detects Histone H1 protein by western blot analysis. Mouse tissue extracts (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with Histone H1 antibody (GTX114462) diluted by 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169