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Histone H1S35ph (phospho Ser35) antibody

Orthogonal
Anti-Histone H1S35ph (phospho Ser35) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121669
Anti-Histone H1S35ph (phospho Ser35) antibody used in IHC (Paraffin sections) (IHC-P). GTX121669
Anti-Histone H1S35ph (phospho Ser35) antibody used in Western Blot (WB). GTX121669

Cat No. GTX121669

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all Histone H1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

Acid-extracted histones from HeLa cell treated Nocodazole

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser35 of human Histone H1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721935

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H1 histone family antibody , Histone H1.4 phospho Ser36 antibody , Phospho Histone H1.4 antibody , Phospho Histone H1.4 (pSer36) antibody , histone H1b antibody , H1E antibody , H1.4S35ph antibody , "histone 1 antibody , Histone H1.4 (phospho Ser36) antibody , member 4 antibody" antibody , "histone cluster 1 antibody , Histone H1.4 phospho S36 antibody , Histone H1.4 (pS36) antibody , Phospho Histone H1.4 (Ser36) antibody , H1.4 antibody , H1F4 antibody , dJ221C16.5 antibody , MGC116819 antibody , Histone H1.4 (phospho S36) antibody , H1e antibody" antibody , Histone H1.4 (pSer36) antibody , Phospho Histone H1.4 (pS36) antibody , histone H1.4 antibody , HIST1H1E antibody

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-Histone H1S35ph (phospho Ser35) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121669

GTX121669 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Histone H1S35ph (phospho Ser35)(GTX121669) antibody at 1:500 dilution.

Anti-Histone H1S35ph (phospho Ser35) antibody used in IHC (Paraffin sections) (IHC-P). GTX121669

GTX121669 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Histone H1S35ph (phospho Ser35)(GTX121669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H1S35ph (phospho Ser35) antibody used in Western Blot (WB). GTX121669

GTX121669 WB Image

Orthogonal

Sample (5 ug of whole cell lysate)
A: Acid-extracted histones from HeLa cell
B: Acid-extracted histones from HeLa cell treated Nocodazole
12% SDS PAGE
GTX121669 diluted at 1:1000

REFERENCE

Application Reference

Krishnan S et al. Mol Cell 2017; 67 (4) : 579-593.e6 Phospho-H1 Decorates the Inter-chromatid Axis and Is Evicted along with Shugoshin by SET during Mitosis.
Application : WB
Reactivity : Human
Chu CS et al. J Biol Chem 2011; 286 (41) : 35843-51 Protein kinase A-mediated serine 35 phosphorylation dissociates histone H1.4 from mitotic chromosome.
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169