GeneTex

  • Country / Location Selection

United States (US)

Histone H1S35ph (phospho Ser35) antibody

Orthogonal
Anti-Histone H1S35ph (phospho Ser35) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121669
Anti-Histone H1S35ph (phospho Ser35) antibody used in Western Blot (WB). GTX121669
Anti-Histone H1S35ph (phospho Ser35) antibody used in IHC (Paraffin sections) (IHC-P). GTX121669

Cat. No. GTX121669

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Histone H1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Positive Control

Acid-extracted histones from HeLa cell treated Nocodazole

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser35 of human Histone H1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10721935

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

DATA IMAGES

Anti-Histone H1S35ph (phospho Ser35) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121669

GTX121669 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Histone H1S35ph (phospho Ser35)(GTX121669) antibody at 1:500 dilution.

Anti-Histone H1S35ph (phospho Ser35) antibody used in Western Blot (WB). GTX121669

GTX121669 WB Image

Orthogonal

Sample (5 ug of whole cell lysate)
A: Acid-extracted histones from HeLa cell
B: Acid-extracted histones from HeLa cell treated Nocodazole
12% SDS PAGE
GTX121669 diluted at 1:1000

Anti-Histone H1S35ph (phospho Ser35) antibody used in IHC (Paraffin sections) (IHC-P). GTX121669

GTX121669 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using Histone H1S35ph (phospho Ser35)(GTX121669) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Krishnan S et al. Mol Cell 2017; 67 (4) : 579-593.e6 Phospho-H1 Decorates the Inter-chromatid Axis and Is Evicted along with Shugoshin by SET during Mitosis.
Application : WB
Reactivity : Human
Chu CS et al. J Biol Chem 2011; 286 (41) : 35843-51 Protein kinase A-mediated serine 35 phosphorylation dissociates histone H1.4 from mitotic chromosome.
Reactivity : Human
Submit a Reference

REVIEW

There are currently no reviews for Histone H1S35ph (phospho Ser35) antibody (GTX121669). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169