Dotblot analysis of anti-Histone H2AK5ac (acetyl Lys5) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) 1:5000 dilution.
A: Peptide samples of H2A.2K5 un
B: Peptide samples of H2A.2K5ac
C: Peptide samples of H2BK20 un
D: Peptide samples of H2BK20ac
E: Peptide samples of H2BK5 un
F: Peptide samples of H2BK5ac

Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein at nucleus by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 0.4 μM TSA for 18 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H2AK5ac (acetyl Lys5) protein stained by Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Histone H2AK5ac (acetyl Lys5) antibody immunoprecipitates Histone H2AK5ac (acetyl Lys5) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H2AK5ac (acetyl Lys5) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H2AK5ac (acetyl Lys5) protein by 2.5 μg of Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) 12% SDS-PAGE The immunoprecipitated Histone H2AK5ac (acetyl Lys5) protein was detected by Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) diluted at 1 : 10000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Histone H2AK5ac (acetyl Lys5) antibody detects Histone H2AK5ac (acetyl Lys5) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Trichostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H2AK5ac (acetyl Lys5) antibody (GTX128961) dilution: 1:10000