WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60814 Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade.
Dilution : 1:500

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H3K18 using GTX60814 Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade.
Dilution : 1:5,000

ChIP analysis of HeLa cells treated with TSA using GTX60814 Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the peak distribution along the complete human X-chromosome and a zoomin to a 600 kb region (figure 2A and B), and in two regions on chromosome 14 and 3 surrounding the c-fos and EIF4A2 positive control genes (figure 2C and D, respectively).
Antibody amount : 1μg

ELISA analysis of peptide containing the histone modification of interest using GTX60814 Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade.

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60814 Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade.
Green : Primary antibody
Blue : DAPI
Dilution : 1:200

ChIP analysis of sheared chromatin from 10⁶ HeLa cells treated with TSA using GTX60814 Histone H3K18ac (Acetyl Lys18) antibody - ChIP grade. A titration consisting of 1, 2, 5 and 10 μg of antibody per ChIP experiment was analyzed. IgG (2 μg/IP) was used as a negative IP control. Quantitative PCR was performed with primers for the promoters of the active EIF4A2 and c-fos genes, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat, used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).