Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27ac (Acetyl Lys27) antibody (GTX128944) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27ac (Acetyl Lys27) antibody (GTX128944) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Dotblot analysis of anti-Histone H3 (acetyl Lys27) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys27) antibody (GTX128944) 1:5000 dilution.
A: Peptide samples of H3K18 un
B: Peptide samples of H3K18ac
C: Peptide samples of H3K9un
D: Peptide samples of H3K9ac
E: Peptide samples of H3K14un
F: Peptide samples of H3K14ac
G: Peptide samples of H3K27un
H: Peptide samples of H3K27ac

Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-Histone H3K27ac (acetyl Lys27) antibody. The precipitated DNA was detected by PCR with primer set targeting to RPL10 promoter or β-globin promoter.

Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H3K27ac (acetyl Lys27) antibody (GTX128944) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H3 (acetyl K27) antibody immunoprecipitates Histone H3 (acetyl K27) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H3 (acetyl K27) protein expressing HeLa cells (0.4μM TSA treatment for 18hr). B : Control with 2.5 μg of pre-immune rabbit IgG. C : Immunoprecipitation of Histone H3 (acetyl K27) protein by 2.5 μg of Histone H3 (acetyl K27) antibody (GTX128944). 12% SDS-PAGE The immunoprecipitated Histone H3 (acetyl K27) protein was detected by Histone H3 (acetyl K27) antibody (GTX128944) diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Histone H3K27ac (acetyl Lys27) antibody (GTX128944) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H3K27ac (acetyl Lys27) antibody (GTX128944) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H3 (acetyl Lys27) antibody detects Histone H3 (acetyl Lys27) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 (acetyl Lys27) protein stained by Histone H3 (acetyl Lys27) antibody (GTX128944) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.