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Histone H3K27ac (Acetyl Lys27) antibody

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Western Blot (WB). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Immunoprecipitation (IP). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in ChIP assay (ChIP assay). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Dot blot (Dot). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX128944

Cat No. GTX128944

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, Dot, ChIP assay, EM

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:5000
Dot Assay dependent
ChIP assay Assay dependent
EM Assay dependent
Not tested in other applications.

Positive Control

HeLa

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3K27ac (acetyl Lys27). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Western Blot (WB). GTX128944

GTX128944 WB Image

Histone H3 (acetyl Lys27) antibody detects Histone H3 (acetyl Lys27) protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Trichostatin A treatment for 18 hr)
15% SDS-PAGE
Histone H3 (acetyl Lys27) antibody (GTX128944) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Immunoprecipitation (IP). GTX128944

GTX128944 IP Image

Histone H3 (acetyl K27) antibody immunoprecipitates Histone H3 (acetyl K27) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H3 (acetyl K27) protein expressing HeLa cells (0.4μM TSA treatment for 18hr). B : Control with 2.5 μg of pre-immune rabbit IgG. C : Immunoprecipitation of Histone H3 (acetyl K27) protein by 2.5 μg of Histone H3 (acetyl K27) antibody (GTX128944). 12% SDS-PAGE The immunoprecipitated Histone H3 (acetyl K27) protein was detected by Histone H3 (acetyl K27) antibody (GTX128944) diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX128944

GTX128944 IHC-P Image

Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H3K27ac (acetyl Lys27) antibody (GTX128944) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128944

GTX128944 ICC/IF Image

Histone H3 (acetyl Lys27) antibody detects Histone H3 (acetyl Lys27) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 (acetyl Lys27) protein stained by Histone H3 (acetyl Lys27) antibody (GTX128944) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in ChIP assay (ChIP assay). GTX128944

GTX128944 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-Histone H3K27ac (acetyl Lys27) antibody. The precipitated DNA was detected by PCR with primer set targeting to RPL10 promoter or β-globin promoter.

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX128944

GTX128944 IHC-P Image

Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Histone H3K27ac (acetyl Lys27) antibody (GTX128944) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Dot blot (Dot). GTX128944

GTX128944 Dot Image

Dotblot analysis of anti-Histone H3 (acetyl Lys27) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys27) antibody (GTX128944) 1:5000 dilution.
A: Peptide samples of H3K18 un
B: Peptide samples of H3K18ac
C: Peptide samples of H3K9un
D: Peptide samples of H3K9ac
E: Peptide samples of H3K14un
F: Peptide samples of H3K14ac
G: Peptide samples of H3K27un
H: Peptide samples of H3K27ac

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX128944

GTX128944 IHC-P Image

Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H3K27ac (acetyl Lys27) antibody (GTX128944) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

Histone H3K27ac (Acetyl Lys27) antibody Cat No. GTX128944

Rating ( Average 3 based on 1 users reviews)
Application
Electron microscopy(EM) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 27-Mar-2017
Score :
Application Tested : EM
Sample Species : Ms
Sample : liver tissue
Blocking : Normal Goat Serum in PBS, 25°C, 0.05Hr
Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2)
Permeabilization /Antigen retrieval : 0.1% BSA in PBST
Primary Antibody : 1:10, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169