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Histone H3K27ac (Acetyl Lys27) antibody

Orthogonal
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Immunoprecipitation (IP). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in ChIP assay (ChIP assay). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Western Blot (WB). GTX128944
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Dot blot (Dot). GTX128944

Cat. No. GTX128944

Reference 12
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, Dot, ChIP assay, EM

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Histone H3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:5000
Dot Assay dependent
ChIP assay Assay dependent
EM Assay dependent
Not tested in other applications.

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys27 of human Histone H3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885844

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128944

GTX128944 ICC/IF Image

Histone H3 (acetyl Lys27) antibody detects Histone H3 (acetyl Lys27) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 (acetyl Lys27) protein stained by Histone H3 (acetyl Lys27) antibody (GTX128944) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX128944

GTX128944 IHC-P Image

Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H3K27ac (acetyl Lys27) antibody (GTX128944) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX128944

GTX128944 IHC-P Image

Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Histone H3K27ac (acetyl Lys27) antibody (GTX128944) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Immunoprecipitation (IP). GTX128944

GTX128944 IP Image

Histone H3 (acetyl K27) antibody immunoprecipitates Histone H3 (acetyl K27) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H3 (acetyl K27) protein expressing HeLa cells (0.4μM TSA treatment for 18hr). B : Control with 2.5 μg of pre-immune rabbit IgG. C : Immunoprecipitation of Histone H3 (acetyl K27) protein by 2.5 μg of Histone H3 (acetyl K27) antibody (GTX128944). 12% SDS-PAGE The immunoprecipitated Histone H3 (acetyl K27) protein was detected by Histone H3 (acetyl K27) antibody (GTX128944) diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in IHC (Paraffin sections) (IHC-P). GTX128944

GTX128944 IHC-P Image

Histone H3K27ac (acetyl Lys27) antibody detects Histone H3K27ac (acetyl Lys27) protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H3K27ac (acetyl Lys27) antibody (GTX128944) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in ChIP assay (ChIP assay). GTX128944

GTX128944 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-Histone H3K27ac (acetyl Lys27) antibody. The precipitated DNA was detected by PCR with primer set targeting to RPL10 promoter or β-globin promoter.

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Western Blot (WB). GTX128944

GTX128944 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27ac (Acetyl Lys27) antibody (GTX128944) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Dot blot (Dot). GTX128944

GTX128944 Dot Image

Dotblot analysis of anti-Histone H3 (acetyl Lys27) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H3 (acetyl Lys27) antibody (GTX128944) 1:5000 dilution.
A: Peptide samples of H3K18 un
B: Peptide samples of H3K18ac
C: Peptide samples of H3K9un
D: Peptide samples of H3K9ac
E: Peptide samples of H3K14un
F: Peptide samples of H3K14ac
G: Peptide samples of H3K27un
H: Peptide samples of H3K27ac

REFERENCE

Application Reference

Sun Y et al. Heliyon 2024; 10 (6) : e27727 EP300 regulates the SLC16A1-AS1-AS1/TCF3 axis to promote lung cancer malignancies through the Wnt signaling pathway.
Y Sun et al. Heliyon 2024; S2405-8440 (24) : 03758-7 EP300 regulates the SLC16A1-AS1-AS1/TCF3 axis to promote lung cancer malignancies through the Wnt signaling pathway
Application : ChIP assay
Zhong X et al. Acta Pharm Sin B 2024; 14 (2) : 712-728 The lysine methyltransferase SMYD2 facilitates neointimal hyperplasia by regulating the HDAC3-SRF axis.
J Shi et al. Mol Med 2023; 29 (1) : 91 Histone acetyltransferase P300 deficiency promotes ferroptosis of vascular smooth muscle cells by activating the HIF-1α/HMOX1 axis
Application : WB
Reactivity : Human
Zhou H et al. EMBO Rep 2022; 23 (5) : e53475 A peptide encoded by pri-miRNA-31 represses autoimmunity by promoting T(reg) differentiation.
Application : ChIP assay
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Submit a Reference

REVIEW

Histone H3K27ac (Acetyl Lys27) antibody Cat. No. GTX128944

Rating
( Average 3 based on 1 users reviews)
Application
Electron microscopy(EM)
( Average 3 based on 1 users reviews)
Anti-Histone H3K27ac (Acetyl Lys27) antibody used in Electron microscopy (EM). GTX128944
Date : Anonymous submitted on 27-Mar-2017
Score :
Application Tested : EM
Sample Species : Ms
Sample : liver tissue
Blocking : Normal Goat Serum in PBS, 25°C, 0.05Hr
Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2)
Permeabilization /Antigen retrieval : 0.1% BSA in PBST
Primary Antibody : 1:10, 4°C, 16Hr
Submit a Review
Application Reference
Sun Y et al. Heliyon 2024; 10 (6):e27727 EP300 regulates the SLC16A1-AS1-AS1/TCF3 axis to promote lung cancer malignancies through the Wnt signaling pathway.
Y Sun et al. Heliyon 2024; S2405-8440 (24):03758-7 EP300 regulates the SLC16A1-AS1-AS1/TCF3 axis to promote lung cancer malignancies through the Wnt signaling pathway
Application : ChIP assay
Zhong X et al. Acta Pharm Sin B 2024; 14 (2):712-728 The lysine methyltransferase SMYD2 facilitates neointimal hyperplasia by regulating the HDAC3-SRF axis.
J Shi et al. Mol Med 2023; 29 (1):91 Histone acetyltransferase P300 deficiency promotes ferroptosis of vascular smooth muscle cells by activating the HIF-1α/HMOX1 axis
Application : WB
Reactivity : Human
Zhou H et al. EMBO Rep 2022; 23 (5):e53475 A peptide encoded by pri-miRNA-31 represses autoimmunity by promoting T(reg) differentiation.
Application : ChIP assay
Li M et al. Bioengineered 2022; 13 (4):11320-11341 GATA binding protein 1 recruits histone deacetylase 2 to the promoter region of nuclear receptor binding protein 2 to affect the tumor microenvironment and malignancy of thyroid carcinoma.
Application : ChIP assay
Reactivity : Human
Terada CI et al. ESC Heart Fail 2022; 9 (5):3031-3043 Histopathological and epigenetic changes in myocardium associated with cancer therapy-related cardiac dysfunction.
Lin Li et al. Cell Death Dis 2021; 12 (8):777 Aberrant super-enhancer-driven oncogene ENC1 promotes the radio-resistance of breast carcinoma.
Application : ChIP assay
Reactivity : Human
Patil S et al. Cells 2021; 10 (12) Chromatin-Independent Interplay of NFATc1 and EZH2 in Pancreatic Cancer.
Application : ChIP assay
Reactivity : Human
Deng R et al. Mol Cancer 2020; 19 (1):122 Disruption of super-enhancer-driven tumor suppressor gene RCAN1.4 expression promotes the malignancy of breast carcinoma.
Application : IP
Reactivity : Human
Olley G et al. Nat Genet 2018; 50 (3):329-332 BRD4 interacts with NIPBL and BRD4 is mutated in a Cornelia de Lange-like syndrome.
Fan CS et al. Oncotarget 2018; 9 (4):4998-5015 Osteopontin-integrin engagement induces HIF-1α-TCF12-mediated endothelial-mesenchymal transition to exacerbate colorectal cancer.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169