Histone H3 (tri-Methyl Lys27) antibody detects Histone H3 (tri-Methyl Lys27) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3 (tri-Methyl Lys27) protein stained by Histone H3 (tri-Methyl Lys27) antibody (GTX129774) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K27me3 (tri-Methyl Lys27) antibody (GTX129774) diluted at 1:1000.

Histone H3 (tri-Methyl Lys27) antibody detects Histone H3 (tri-Methyl Lys27) protein at nucleus on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H3 (tri-Methyl Lys27) antibody (GTX129774) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H3 (tri-Methyl Lys27) antibody detects Histone H3 (tri-Methyl Lys27) protein at nucleus on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H3 (tri-Methyl Lys27) antibody (GTX129774) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H3 (tri-Methyl Lys27) antibody detects Histone H3 (tri-Methyl Lys27) protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys27) antibody (GTX129774) diluted by 1:1000.