The GTX60895 reacts to Histone H3 dimethylated at Lysine 79 (K79me2). Very slightly cross reactivity with monomethylated Lysine 14 (K14me1), and no cross reactivity with non-modified Lysine 79 (K79 ctrl), trimethylated Lysine 79 (K79me3), or other methylations in Histone H3.

ICC/IF analysis of HepG2 cells using GTX60895 Histone H3K79me2 (Di-methyl Lys79) antibody [RM181].

WB analysis of recombinant histone H3.3 (1) and acid extracts of HeLa cells (2) using GTX60895 Histone H3K79me2 (Di-methyl Lys79) antibody [RM181].
Dilution : 0.25μg/ml

ChIP analysis of HeLa cells using GTX60895 Histone H3K79me2 (Di-methyl Lys79) antibody [RM181]. Real-time PCR was performed using primers specific to the gene indicated.
Antibody amount : 5μg