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Histone H3K9acS10ph (Acetyl Lys9/phospho Ser10) antibody [APH3-64]

Cat No. GTX12180

Host

Mouse

Clonality

Monoclonal

Clone Name

APH3-64

Isotype

IgG2a

Application

WB, IP, ELISA

Reactivity

Human, Mouse, Rat, Drosophila, Bovine, Chicken, Caenorhabditis elegans, Frog
Package
50 μl ($289)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

Jurkat treated with Nocodazole

PROPERTIES

Form

Liquid

Buffer

0.01M PBS pH7.4

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

~2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

synthetic, acetylated and phosphorylated histone H3 peptide (amino acids 7-20, Ac-Lys9, pSer10) corresponding to the N-terminus of human histone H3. The sequence is identical in many species.

Purification

Purified immunoglobulin

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H3 histone family member 3A , H3.3A , H3F3

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fibre is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.Covalent modifications of the canonical core histones, including acetylation, phosphorylation, methylation, and monoubiquitination, are used to mark nucleosomes to create chromatin domains with a range of functions. The information encoded by the modifications can contribute to the formation and/or maintenance of transcriptionally active and inactive chromatin in response to various signalling pathways. Phosphorylation, particularly that of histones H1 and H3, has long been implicated in chromosome condensation during mitosis. However, converging evidence suggests that H3 phosphorylation (specifically serine 10) is also directly correlated with the induction of immediate-early genes such as c-jun, c-fos and c-myc. The potential importance of the serine 10 phosphorylation mark in H3 is strengthened by the finding that MSK1, a kinase activated by growth factor and stress stimuli, also phosphorylates H3 in vitro. H3 phosphorylation at serine 10 in conjunction with phosphorylation at serine 28, is also required for proper segregation and condensation of chromosomes during mitosis and meiosis.

Database

Research Area

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 289