GeneTex
  • Country / Location Selection

United States (US)

Histone H3K9me3 (Tri-methyl Lys9) antibody

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Western Blot (WB). GTX121677
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in ChIP assay (ChIP assay). GTX121677
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX121677
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Immunoprecipitation (IP). GTX121677
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX121677
Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121677

Cat No. GTX121677

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, ChIP assay, EM

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
ChIP assay Assay dependent
EM Assay dependent
Not tested in other applications.

Calculated MW

15 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H3K9me3 (trimethyl Lys9). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Cellular Localization

Nucleus

Research Area

DATA IMAGES

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Western Blot (WB). GTX121677

GTX121677 WB Image

Histone H3 (tri-Methyl Lys9) antibody detects Histone H3 (tri-Methyl Lys9) protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3 (tri-Methyl Lys9) antibody (GTX121677) diluted at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in ChIP assay (ChIP assay). GTX121677

GTX121677 ChIP assay Image

Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-Histone H3K9me3 (trimethyl Lys9) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX121677

GTX121677 IHC-P Image

Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Histone H3K9me3 (trimethyl Lys9) antibody (GTX121677) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Immunoprecipitation (IP). GTX121677

GTX121677 IP Image

Immunoprecipitation of Histone H3 (tri-Methyl Lys9) protein from 293T whole cell extracts using 5 μg of Histone H3 (tri-Methyl Lys9) (GTX121677).
Western blot analysis was performed using Histone H3 (tri-Methyl Lys9) (GTX121677).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in IHC (Paraffin sections) (IHC-P). GTX121677

GTX121677 IHC-P Image

Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3K9me3 (trimethyl Lys9) antibody (GTX121677) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Histone H3K9me3 (Tri-methyl Lys9) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX121677

GTX121677 ICC/IF Image

Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K9me3 (trimethyl Lys9) protein stained by Histone H3K9me3 (trimethyl Lys9) antibody (GTX121677) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

REVIEW

Histone H3K9me3 (Tri-methyl Lys9) antibody Cat No. GTX121677

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Electron microscopy(EM) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:100, 4°C, 16Hr
Lane Description : Lane 1: MG63 whole cell lysate, Lane 2: doxorubicin resistant MG63 cell line, Lane 3: Cisplatin resistant MG63 cell line
Date : Anonymous submitted on 27-Mar-2017
Score :
Application Tested : EM
Sample Species : Ms
Sample : liver tissue
Blocking : Normal Goat Serum in PBS, 25°C, 0.08Hr
Fixation : 4% PFA in S?rensen phosphate buffer (pH 7.2) or in culture medium
Permeabilization /Antigen retrieval : 0.1% BSA in PBST
Primary Antibody : 1:10, 4°C, 16Hr
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169