Histone H3K9me3 (Tri-methyl Lys9) antibody

Immunoprecipitation of Histone H3 (tri-Methyl Lys9) protein from 293T whole cell extracts using 5 μg of Histone H3 (tri-Methyl Lys9) (GTX121677).
Western blot analysis was performed using Histone H3 (tri-Methyl Lys9) (GTX121677) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3K9me3 (trimethyl Lys9) protein stained by Histone H3K9me3 (trimethyl Lys9) antibody (GTX121677) diluted at 1:500.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Cross-linked ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-Histone H3K9me3 (trimethyl Lys9) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.

Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3K9me3 (trimethyl Lys9) antibody (GTX121677) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H3K9me3 (trimethyl Lys9) antibody detects Histone H3K9me3 (trimethyl Lys9) protein at nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
Histone H3K9me3 (trimethyl Lys9) antibody (GTX121677) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Histone H3K9me3 (Tri-methyl Lys9) antibody (GTX121677) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.