Histone H3S10ph (phospho Ser10) antibody [GT921] detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [GT921] (GTX630185) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H3S10ph (phospho Ser10) antibody [GT921] detects Histone H3S10ph (phospho Ser10) protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Histone H3S10ph (phospho Ser10) antibody [GT921] (GTX630185) diluted at 1:250.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Histone H3S10ph (phospho Ser10) antibody [GT921] detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunofluorescent analysis.
Samples: HCT116 cells mock (left) and treated with 30 μM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: p21 Cip1 protein stained by p21 Cip1 antibody (GTX100444) diluted at 1:1000.
Red: Histone H3S10ph (phospho Ser10) protein stained by Histone H3S10ph (phospho Ser10) antibody [GT921] (GTX630185) diluted at 1:500.
Blue: Hoechst 33342 staining.

Histone H3 (phospho Ser10) antibody [GT921] detects Histone H3 (phospho Ser10) protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (untreated) |
B. 30 μg HeLa whole cell lysate/extract (100 ng/ml Nocodazole treatment for 24 hr)
15 % SDS-PAGE
Histone H3 (phospho Ser10) antibody [GT921] (GTX630185) dilution: 1:1000

Histone H3S10ph (phospho Ser10) antibody [GT921] detects Histone H3S10ph (phospho Ser10) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Histone H3S10ph (phospho Ser10) stained by Histone H3S10ph (phospho Ser10) antibody [GT921] (GTX630185) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min