Dot blot analysis of various of H4K20 methylation peptides using Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (GTX630545) at antibody dilution of 1:500.
Un : H4 protein peptide
Me : H4K20 mono-methyl peptide
2Me : H4K20 di-methyl peptide
3Me : H4K20 Tri-methyl peptide

Histone H4 (di-Methyl Lys20) antibody [GT1851] detects Histone H4 (di-Methyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 (di-Methyl Lys20) protein stained by Histone H4 (di-Methyl Lys20) antibody [GT1851] (GTX630545) diluted at 1:500.
Blue: Hoechst 33342 staining.

Immunoprecipitation of HIST1H4A protein from HeLa whole cell extracts using 5 μg of Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (GTX630545).
Western blot analysis was performed using Histone H4K20me2 (dimethyl Lys20) antibody [GT1851] (GTX630545).
EasyBlot anti-mouse IgG (GTX221667-01) was used as a secondary reagent.

Histone H4 (di methyl Lys20) antibody [GT1851] detects Histone H4 (di methyl Lys20) protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
Histone H4 (di methyl Lys20) antibody [GT1851] (GTX630545) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Cross-linked ChIP was performed with HeLa chromatin extract and 5 μg of either control mouse IgG or anti-Histone H4K20me2 (dimethyl Lys20) antibody. The precipitated DNA was detected by PCR with primer set targeting to MyoD.