Dot blot analysis of various of H4K20 methylation peptides using Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) at antibody dilution of 1:500.
Un : H4 protein peptide
Me : H4K20 mono-methyl peptide
2Me : H4K20 di-methyl peptide
3Me : H4K20 Tri-methyl peptide

Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 (tri methyl Lys20) protein stained by Histone H4 (tri methyl Lys20) antibody (GTX128960) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.

Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse muscle by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Histone H4 (tri methyl Lys20) antibody detects Histone H4 (tri methyl Lys20) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
15 % SDS-PAGE
Histone H4 (tri methyl Lys20) antibody (GTX128960) dilution: 1:2000

Histone H4K20me3 (trimethyl Lys20) antibody detects Histone H4K20me3 (trimethyl Lys20) protein at nucleus on mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Histone H4K20me3 (trimethyl Lys20) antibody (GTX128960) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min