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Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade

Anti-Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60337
Anti-Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade used in Dot blot (Dot). GTX60337
Anti-Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade used in Western Blot (WB). GTX60337
Anti-Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade used in ELISA (ELISA). GTX60337
Anti-Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60337
Anti-Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60337

Cat No. GTX60337

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, Dot, ELISA, ChIP assay

Reactivity

Human, Mouse
Package
50 μg ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1,000
ICC/IF 1:500
Dot 1:20,000
ELISA 1:1,000
ChIP assay 0.5-5 μg
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% sodium azide, 0.05% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.76 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The region of histone H4 containing the acetylated lysines 5, 8 and 12 (H4K5,8,12ac), using a KLH-conjugated synthetic peptide.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60337

GTX60337 ChIP assay Image

ChIP analysis of sheared chromatin from 1x10⁵ K562 cells using GTX60337 Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade. The IP'd DNA was subsequently analysed on an Illumina Genome Analyzer. Library preparation, cluster generation and sequencing were performed according to the manufacturer's instructions. The 36 bp tags were aligned to the human genome using the ELAND algorithm. Figure 2 shows the signal distribution along the complete length of chromosome 2 (figure 2A) and a zoomin to a 600 kb region (figure 2B). Figure 2C and D show the enrichment in two genomic regions on chromosome 3 and 12, respectively, containing EIF4A2 and GAPDH positive controls.
Antibody amount : 0.5μg

Anti-Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade used in Dot blot (Dot). GTX60337

GTX60337 Dot Image

Dot blot analysis of 0.2 - 100 pmol of the peptides containing other histone modifications and the unmodified H4 using GTX60337 Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade.
Dilution : 1:20,000

Anti-Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade used in Western Blot (WB). GTX60337

GTX60337 WB Image

WB analysis of whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using GTX60337 Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade.
Dilution : 1:1,000

Anti-Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade used in ELISA (ELISA). GTX60337

GTX60337 ELISA Image

ELISA analysis of peptide containing the histone modification of interest using GTX60337 Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade.

Anti-Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade used in ChIP assay (ChIP assay). GTX60337

GTX60337 ChIP assay Image

ChIP analysis of sheared chromatin from 10⁶ K562 cells using GTX60337 Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade. A titration of the antibody consisting of 0.2, 0.5, 1 and 2 μg per ChIP experiment was analysed. IgG (1 μg/IP) was used as negative IP control. QPCR was performed with primers for promoter of the active gene EIF4A2 and for a region 1 kb upstream of the GAPDH gene, used as positive controls, and for the inactive MYOD1 gene and the Sat2 satellite repeat region used as negative controls. This figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

Anti-Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60337

GTX60337 ICC/IF Image

ICC/IF analysis of 4% paraformaldehyde fixed HeLa cells using GTX60337 Histone H4K5K8K12ac (acetyl Lys5/Lys8/Lys12) antibody - ChIP grade. Staining of the cells with the H4K5,8,12ac antibody after incubation of the antibody with 10 ng/μl of the following blocking peptides: H4K5,8,12 unmodified (figure B), H4K5,8,12ac (figure C), H2A.ZK5,7,11ac (figure D) and H4K5,8,12,16ac (figure E).
Green : Primary antibody
Blue : DAPI
Dilution : 1:500

REFERENCE

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REVIEW

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