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Histone H4K8ac (acetyl Lys8) antibody

Anti-Histone H4K8ac (acetyl Lys8) antibody used in IHC (Paraffin sections) (IHC-P). GTX128957
Anti-Histone H4K8ac (acetyl Lys8) antibody used in Dot blot (Dot). GTX128957
Anti-Histone H4K8ac (acetyl Lys8) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128957
Anti-Histone H4K8ac (acetyl Lys8) antibody used in IHC (Paraffin sections) (IHC-P). GTX128957
Anti-Histone H4K8ac (acetyl Lys8) antibody used in ChIP assay (ChIP assay). GTX128957
Anti-Histone H4K8ac (acetyl Lys8) antibody used in Western Blot (WB). GTX128957
Anti-Histone H4K8ac (acetyl Lys8) antibody used in Immunoprecipitation (IP). GTX128957
Anti-Histone H4K8ac (acetyl Lys8) antibody used in IHC (Paraffin sections) (IHC-P). GTX128957

Cat No. GTX128957

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IP, Dot, ChIP assay

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:5000
Dot Assay dependent
ChIP assay Assay dependent
Not tested in other applications.

Positive Control

HeLa

Predict Reactivity

Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Histone H4K8ac (acetyl Lys8). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

H4FA Antibody, histone cluster 1, H4a Antibody, HIST1H4A Antibody

Cellular Localization

Nucleus

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Research Area

DATA IMAGES

Anti-Histone H4K8ac (acetyl Lys8) antibody used in IHC (Paraffin sections) (IHC-P). GTX128957

GTX128957 IHC-P Image

Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H4K8ac (acetyl Lys8) antibody (GTX128957) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H4K8ac (acetyl Lys8) antibody used in Dot blot (Dot). GTX128957

GTX128957 Dot Image

Dotblot analysis of anti-Histone H4 (acetyl Lys9) antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with Histone H4 (acetyl Lys9) antibody (GTX128957) 1:5000 dilution.
A: Peptide samples of H4K8 un
B: Peptide samples of H4K8ac
C: Peptide samples of H4K16un
D: Peptide samples of H4K16ac

Anti-Histone H4K8ac (acetyl Lys8) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX128957

GTX128957 ICC/IF Image

Histone H4 (acetyl Lys8) antibody detects Histone H4 (acetyl Lys8) protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H4 (acetyl Lys8) protein stained by Histone H4 (acetyl Lys8) antibody (GTX128957) diluted at 1:500.
Red: alpha Tubulin antibody [GT114], a cytoskeleton marker, stained by GTX628802 diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Histone H4K8ac (acetyl Lys8) antibody used in IHC (Paraffin sections) (IHC-P). GTX128957

GTX128957 IHC-P Image

Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on mouse kidney by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Histone H4K8ac (acetyl Lys8) antibody (GTX128957) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Histone H4K8ac (acetyl Lys8) antibody used in ChIP assay (ChIP assay). GTX128957

GTX128957 ChIP assay Image

Cross-linked ChIP was performed with HeLa chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-Histone H4K8ac (acetyl Lys8) antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or TEXT12.

Anti-Histone H4K8ac (acetyl Lys8) antibody used in Western Blot (WB). GTX128957

GTX128957 WB Image

Histone H4 (acetyl Lys8) antibody detects Histone H4 (acetyl Lys8) protein by Western blot analysis.
A. 30 μg HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 μg HeLa whole cell lysate/extract (0.4 μM Trichostatin A treatment for 18 hr)
15 % SDS-PAGE
Histone H4 (acetyl Lys8) antibody (GTX128957) dilution: 1:5000

Anti-Histone H4K8ac (acetyl Lys8) antibody used in Immunoprecipitation (IP). GTX128957

GTX128957 IP Image

Histone H4 (acetyl K9) antibody immunoprecipitates Histone H4 (acetyl K9) protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 μg whole cell lysate/extract of Histone H4 (acetyl K9) protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of Histone H4 (acetyl K9) protein by 2.5 μg of Histone H4 (acetyl K9) antibody (GTX128957). 12% SDS-PAGE The immunoprecipitated Histone H4 (acetyl K9) protein was detected by Histone H4 (acetyl K9) antibody (GTX128957) diluted at 1 : 5000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Histone H4K8ac (acetyl Lys8) antibody used in IHC (Paraffin sections) (IHC-P). GTX128957

GTX128957 IHC-P Image

Histone H4K8ac (acetyl Lys8) antibody detects Histone H4K8ac (acetyl Lys8) protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
Histone H4K8ac (acetyl Lys8) antibody (GTX128957) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169