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Hsp70 antibody

Comparison
Anti-Hsp70 antibody used in Immunoprecipitation (IP). GTX104126
Anti-Hsp70 antibody used in Western Blot (WB). GTX104126
Anti-Hsp70 antibody used in Western Blot (WB). GTX104126
Anti-Hsp70 antibody used in Western Blot (WB). GTX104126
Anti-Hsp70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104126
Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX104126
Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX104126

Cat. No. GTX104126

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Hsp70 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:30000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:500-1:1000
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Mouse brain , rat brain

Predict Reactivity

Sheep, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSP70. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950519

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heat shock protein family A (Hsp70) member 1A , heat shock protein family A (Hsp70) member 1B , HEL-S-103 , HSP70-1 , HSP70-1A , HSP70-2 , HSP70.1 , HSP70.2 , HSP70I , HSP72 , HSPA1 , HSP70-1 , HSP70-1B , HSP70-2 , HSP70.1 , HSP70.2 , HSP72 , HSPA1 , HSX70

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Hsp70 antibody used in Immunoprecipitation (IP). GTX104126

GTX104126 IP Image

HSP70 1A antibody immunoprecipitates HSP70 1A protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2.5 μg of preimmune rabbit IgG C. Immunoprecipitation of HSP70 1A protein by 2.5 μg of HSP70 1A antibody (GTX104126) 12% SDS-PAGE The immunoprecipitated HSP70 1A protein was detected by HSP70 1A antibody (GTX104126) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Hsp70 antibody used in Western Blot (WB). GTX104126

GTX104126 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HSPA1A antibody (GTX104126) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp70 antibody used in Western Blot (WB). GTX104126

GTX104126 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody (GTX104126) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp70 antibody used in Western Blot (WB). GTX104126

GTX104126 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hsp70 antibody (GTX104126) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Hsp70 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104126

GTX104126 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using HSP70 1A(GTX104126) antibody at 1:200 dilution.

Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX104126

GTX104126 IHC-P Image

HSP70 1A antibody detects HSP70 1A protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
HSP70 1A antibody (GTX104126) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Hsp70 antibody used in IHC (Paraffin sections) (IHC-P). GTX104126

GTX104126 IHC-P Image

HSP70 1A antibody detects HSP70 1A protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
HSP70 1A antibody (GTX104126) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Hong X et al. J Biol Chem 2023; 299 (6) : 104814 Diarylheptanoid 35d overcomes EGFR TKI resistance by inducing hsp70-mediated lysosomal degradation of EGFR in EGFR-mutant lung adenocarcinoma.
Lopez-Toledo G et al. Front Aging Neurosci 2022; 14 : 921573 Patient-Derived Fibroblasts With Presenilin-1 Mutations, That Model Aspects of Alzheimer's Disease Pathology, Constitute a Potential Object for Early Diagnosis.
Application : WB
Reactivity : Human
Fang CT et al. Cell Death Dis 2020; 11 (8) : 715 HSP70 regulates Eg5 distribution within the mitotic spindle and modulates the cytotoxicity of Eg5 inhibitors.
Gueugneau M et al. BMC Genomics 2014; 15 (1) : 1165 Proteomics of muscle chronological ageing in post-menopausal women.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169