Reagent for detecting early stages of apoptosis. During apoptosis, phosphatidylserine (PS) is translocated from the cytoplasmic face of the plasma membrane to the cell surface. Annexin V has a strong, Ca2+-dependent affinity for PS and therefore is used as a probe for detecting apoptosis. The unlabeled Annexin V reagent can be conjugated to any tag of choice for detection of apoptosis. The reagent can also be used in competition binding experiments to compete with tagged annexin V conjugates. Annexin V-FITC, -Cy3, -Cy5, -EGFP, -PE, PE/CY5, and -Biotin Labels are also available separately.
Lyophilized from PBS (pH 7.4). Reconstitute with ddH₂O or PBS to 1.0-5.0 mg/ml.
Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.
≥ 98% by SDS-PAGE and HPLC.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
annexin A5 , ANX5 , ENX2 , HEL-S-7 , PP4 , RPRGL3
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]
Analytical Size Exclusion Chromatography run of Annexin V.