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Human Cathepsin S protein, His tag (active)

Human Cathepsin S protein, His tag (active). GTX01212-pro

Cat No. GTX01212-pro

Application

Functional Assay

Species

Human
Package
20 μg ($329),
10 μg ($249)

APPLICATION

Application Note

1. Measured by its ability to cleave a fluorogenic peptide substrate, (7-methoxycoumarin-4-yl) acetyl-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys (2, 4-dinitrophenyl)-NH2. Cleavage of (7-methoxycoumarin-4-yl) acetyl-Arg-Pro-Lys-Pro-Val-Glu-Nva-Trp-Arg-Lys (2, 4-dinitrophenyl)-NH2 can be measured using excitation and emission wavelength at 320 nm and 405 nm, respectively. The specific activity is > 300 pmoles/min/μg.
Note: The enzyme achieves its activity under acidic pH).
2. Measured by its ability to bind Human Cathepsin S protein (active) (GTX01210-pro) in a functional ELISA.

Observed MW

37 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

C-terminal poly His tagged; Met1-Ile331 of Human Cathepsin S protein (NP_004070.3)

Expression System

HEK293 cells

Purity

> 90% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

CTSS

Background

The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The encoded protein can function as an elastase over a broad pH range in alveolar macrophages. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]

Database

Research Area

DATA IMAGES

Human Cathepsin S protein, His tag (active). GTX01212-pro

GTX01212-pro Image

SDS-PAGE of 5 μg GTX01212-pro Human Cathepsin S protein, His tag (active).

REFERENCE

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REVIEW

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Package List Price ($)
$ 329
$ 249