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Human Cofilin 1 protein, His tag

Human Cofilin 1 protein, His tag. GTX00270-pro
Human Cofilin 1 protein, His tag. GTX00270-pro
Human Cofilin 1 protein, His tag. GTX00270-pro

Cat. No. GTX00270-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($259)
See all Cofilin 1 protein products

APPLICATION

Application Note

CFL1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-CFL1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of CFL1 and ACTb was in a dose dependent manner.

Observed MW

22 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ser3~Leu161 (NP_005498.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

cofilin 1 , CFL , HEL-S-15 , cofilin

Background

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.[supplied by OMIM, Apr 2004]

Database

Research Area

DATA IMAGES

Human Cofilin 1 protein, His tag. GTX00270-pro

GTX00270-pro Functional Assay Image

Functional ELISA analysis of GTX00270-pro Human Cofilin 1 protein which can bind immobilized ACTb protein.

Human Cofilin 1 protein, His tag. GTX00270-pro

GTX00270-pro Image

SDS-PAGE analysis of GTX00270-pro Human Cofilin 1 protein.

Human Cofilin 1 protein, His tag. GTX00270-pro

GTX00270-pro Image

WB analysis of GTX00270-pro Human Cofilin 1 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 259