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Human EPO protein (active)

Human EPO protein (active). GTX00422-pro
Human EPO protein (active). GTX00422-pro
Human EPO protein (active). GTX00422-pro

Cat No. GTX00422-pro

Application Functional Assay
Species Human
Package
10 μg ($259)
APPLICATION

Application Note

Erythropoietin (EPO), also known as hematopoietin or hemopoietin, is a glycoprotein cytokine secreted by the kidney in response to cellular hypoxia; it stimulates red blood cell production (erythropoiesis) in the bone marrow. Erythropoietin is an essential hormone for red blood cell production. EPO can cooperate with various other growth factors involved in the development of erythroid lineage from multipotent progenitors. To test the effect of EPO on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well with 1% serum standard 1640 including various concentrations of recombinant human EPO. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of TF-1 cells after incubation with EPO for 72h observed by inverted microscope, and cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant EPO for 72h. EPO significantly increased cell viability of TF-1 cells.

Observed MW

48 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Reconstitute with 20 mM Tris (pH8.0), 150 mM NaCl to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag and GST-Tag; Ala28~Arg193 (NP_000790.2)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

erythropoietin,DBAL,ECYT5,EP,MVCD2

Background

This gene encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The encoded protein is mainly synthesized in the kidney, secreted into the blood plasma, and binds to the erythropoietin receptor to promote red blood cell production, or erythropoiesis, in the bone marrow. Expression of this gene is upregulated under hypoxic conditions, in turn leading to increased erythropoiesis and enhanced oxygen-carrying capacity of the blood. Expression of this gene has also been observed in brain and in the eye, and elevated expression levels have been observed in diabetic retinopathy and ocular hypertension. Recombinant forms of the encoded protein exhibit neuroprotective activity against a variety of potential brain injuries, as well as antiapoptotic functions in several tissue types, and have been used in the treatment of anemia and to enhance the efficacy of cancer therapies. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES
Human EPO protein (active). GTX00422-pro

GTX00422-pro Functional Assay Image

Cell proliferation effect of GTX00422-pro Human EPO protein (active), cell viability was measured by cell Counting Kit-8 (CCK-8).
(A) Unstimulated TF-1 cells cultured in RPMI-1640 for 72hrs.
(B) TF-1 cells cultured in RPMI-1640, stimulated with 5 ng/ml EPO for 72hrs.

Human EPO protein (active). GTX00422-pro

GTX00422-pro Image

WB analysis of GTX00422-pro Human EPO protein (active).

Human EPO protein (active). GTX00422-pro

GTX00422-pro Image

SDS-PAGE analysis of GTX00422-pro Human EPO protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 259