APPLICATION
Application Note
Measured in a cell proliferation assay using Balb/c 3T3 cells. The ED₅₀ for this effect is typically 0.02-0.1 ng/ml.
Observed MW
16.5 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Batch dependent (please contact us for details)
Storage
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
No tagged; Pro143-Ser288 of Human FGF2 protein (NP_001997.5)
Expression System
E. coli
Purity
> 95% by SDS-PAGE.
Endotoxin
< 1.0 EU/μg (determined by LAL method)
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
BFGF,FGF-2,FGFB,HBGF-2
Background
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
|
GTX01349-pro Image
SDS-PAGE of 5 μg GTX01349-pro Human FGF2 protein (active).
|
|
GTX01349-pro Functional Assay Image
The protein activity of GTX01349-pro Human FGF2 protein was measured in a cell proliferation assay using Balb/c 3T3 cells. ED₅₀ : 0.02-0.1 ng/ml
|
REFERENCE
There are currently no references for Human FGF2 protein (active) (GTX01349-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human FGF2 protein (active) (GTX01349-pro). Be the first to share your experience with this product.