APPLICATION
Application Note
Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to ICAM1-coated plates (12.5 μg/ml, 100 μl/well), approximately 30%-60% will adhere specifically.
Observed MW
65-70 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Batch dependent (please contact us for details)
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Region/Sequence
C-terminal polyhistidine tag; human ICAM1 (NP_000192.2) extracellular domain (Met 1-Glu 480)
Expression System
HEK293 Cells
Purity
>98 % by SDS-PAGE
Endotoxin
<1.0EU/1μg (determined by the LAL method)
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
intercellular adhesion molecule 1 , BB2 , CD54 , P3.58
Cellular Localization
Membrane; Single-pass type I membrane protein
Background
This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX03489-pro Image
SDS-PAGE of GTX03489-pro Human ICAM1 (ECD) protein, His tag (active).
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REFERENCE
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REVIEW
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