APPLICATION
Application Note
Measured by its ability to inhibit rh IFNβ mediated protection of WISH cells infected with VSV (vesicular stomatitis virus) to viral lysis. The EC₅₀ for this effect is typically 0.2-1.2 μg/ml.
2. Measured by its binding ability in a functional ELISA. Immobilized Human Interferon alpha 2 protein (active) (GTX01426-pro) at 10 μg/ml (100 μL/well) can bind Human IFNAR2 protein (active) (GTX01363-pro). The EC₅₀ of Human IFNAR2 protein (active) (GTX01363-pro) is 60-200 ng/ml.
Observed MW
65-75 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Batch dependent (please contact us for details)
Storage
Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
C-terminal human IgG1-Fc tagged; Met1-Lys243 of Human IFNAR2 protein (NP_997468.1)
Expression System
HEK293 cells
Purity
> 90% by SDS-PAGE.
Endotoxin
< 1.0 EU/μg (determined by LAL method)
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
IFN-R,IFN-alpha-REC,IFNABR,IFNARB,IMD45
Background
The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Multiple transcript variants encoding at least two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX01363-pro Image
SDS-PAGE of 5 μg GTX01363-pro Human IFNAR2 protein, human IgG1 Fc tag (active).
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GTX01363-pro Functional Assay Image
The protein binding activity was measured in a functional ELISA analysis of GTX01363-pro Human IFNAR2 protein which can bind immobilized GTX01426-pro Human Interferon alpha 2 protein.
Immobilized GTX01426-pro : 10 μg/ml (100 μl/well)
GTX01363-pro : 60-200 ng/ml
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REFERENCE
There are currently no references for Human IFNAR2 protein, human IgG1 Fc tag (active) (GTX01363-pro). Be the first to share your publications with this product.
REVIEW
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