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Human IL1 Receptor antagonist protein (active)

Human IL1 Receptor antagonist protein (active). GTX00277-pro
Human IL1 Receptor antagonist protein (active). GTX00277-pro
Human IL1 Receptor antagonist protein (active). GTX00277-pro

Cat No. GTX00277-pro

Application Functional Assay
Species Human
Package
10 μg ($239)
APPLICATION

Application Note

IL-1RA is an agent that binds non-productively to the cell surface interleukin-1 receptor (IL-1R), the same receptor that binds interleukin 1 (IL-1), preventing IL-1 from sending a signal to that cell. Besides, Interleukin 1 Alpha (IL-1a) has been identified as an interactor of IL-1RA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL-1RA and recombinant human IL-1a. Briefly, IL1RA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL-1a-coated microtiter wells and incubated for 2 h at 37ºC. Wells were washed with PBST and incubated for 1 h with anti-IL-1RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL-1RA and IL-1a was in a dose dependent manner.

Observed MW

22/25/27 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Arg26~Glu177 (NP_000568.1)

Expression System

293F cell

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 1 receptor antagonist,DIRA,ICIL-1RA,IL-1RN,IL-1ra,IL-1ra3,IL1F3,IL1RA,IRAP,MVCD4

Background

The protein encoded by this gene is a member of the interleukin 1 cytokine family. This protein inhibits the activities of interleukin 1, alpha (IL1A) and interleukin 1, beta (IL1B), and modulates a variety of interleukin 1 related immune and inflammatory responses. This gene and five other closely related cytokine genes form a gene cluster spanning approximately 400 kb on chromosome 2. A polymorphism of this gene is reported to be associated with increased risk of osteoporotic fractures and gastric cancer. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jan 2016]

Database

Research Area

DATA IMAGES
Human IL1 Receptor antagonist protein (active). GTX00277-pro

GTX00277-pro Functional Assay Image

Functional ELISA analysis of GTX00277-pro Human IL1 Receptor antagonist protein (active) which can bind immobilized IL-1 alpha protein.

Human IL1 Receptor antagonist protein (active). GTX00277-pro

GTX00277-pro Image

WB analysis of GTX00277-pro Human IL1 Receptor antagonist protein (active).

Human IL1 Receptor antagonist protein (active). GTX00277-pro

GTX00277-pro Image

SDS-PAGE analysis of GTX00277-pro Human IL1 Receptor antagonist protein (active).

REFERENCE
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REVIEW
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