GeneTex
  • Country / Location Selection

United States (US)

Human IL12A + IL27B fusion protein (active)

Human IL12A + IL27B fusion protein (active). GTX00070-pro
Human IL12A + IL27B fusion protein (active). GTX00070-pro
Human IL12A + IL27B fusion protein (active). GTX00070-pro

Cat No. GTX00070-pro

Application Functional Assay
Species Human
Package
10 μg ($249)
APPLICATION

Application Note

IL35 (Interleukin 35) is an IL-12 family cytokine, which is a dimeric protein composed of IL-12α and IL-27β chains. IL35 is thought to mediate the immune inhibitory function of regulatory T cells and has been proven to promotes pancreas cancer growth through enhancement of proliferation and inhibition of apoptosis. Thus, proliferation assay of IL35 was conducted using PANC-1 cells. Briefly, PANC-1 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM  prior to the addition of various concentrations of recombinant human IL35. After incubated for 48h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of PANC-1 cells after incubation with IIL35 for 48h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with human recombinant IL35 for 48h, and IL35 significantly increased cell viability of PANC-1 cells.

Observed MW

55 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; The IL35 complex composed of IL27B subunit (Arg21~Lys229 of NP_005746.2) and the mature form of human IL12A (Arg23~Ser219 of NP_000873.2) linked by a polypeptide linker (GGGGS)3

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 12A,Epstein-Barr virus induced 3,CLMF,IL-12A,NFSK,NKSF1,P35,IL-27B,IL27B,IL35B

Database

DATA IMAGES
Human IL12A + IL27B fusion protein (active). GTX00070-pro

GTX00070-pro Functional Assay Image

Cell proliferation effect of GTX00070-pro Human IL12A + IL27B fusion protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated PANC-1 cells cultured in DMEM for 48hrs.
(B) PANC-1 cells cultured in DMEM, stimulated with 1000 ng/ml IL35 for 48hrs.

Human IL12A + IL27B fusion protein (active). GTX00070-pro

GTX00070-pro Image

SDS-PAGE analysis of GTX00070-pro Human IL12A + IL27B fusion protein (active).

Human IL12A + IL27B fusion protein (active). GTX00070-pro

GTX00070-pro Image

WB analysis of GTX00070-pro Human IL12A + IL27B fusion protein (active).

REFERENCE
There are currently no references for Human IL12A + IL27B fusion protein (active) (GTX00070-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human IL12A + IL27B fusion protein (active) (GTX00070-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 249