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Human IL17B protein, His tag (active)

Human IL17B protein, His tag (active). GTX00134-pro
Human IL17B protein, His tag (active). GTX00134-pro
Human IL17B protein, His tag (active). GTX00134-pro

Cat. No. GTX00134-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($219)
See all IL17B protein products

APPLICATION

Application Note

IL17B (Interleukin-17B) is is a T cell-derived cytokine that shares sequence similarity with IL17, which has been proven to mediate potent inflammatory immune response. Besides, increasing evidence suggests that IL17 may play a critical role in various kinds of liver diseases, including hepatocellualar carcinoma. Thus a stimulation assay has been conducted to detect the bioactivity of IL17B. HepG2 cells were seeded into 24-well plate at a density of 1×10⁵ cells/ml, and allowed to attach overnight before treated with or without certain concentrations (250 ng/ml, 500 ng/ml) of IL17B for 20h and IL-8 levels in the cell supernatant were determined by ELISA.

Observed MW

20 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gln21~Phe180 (NP_001304916.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 17B , IL-17B , IL-20 , NIRF , ZCYTO7

Background

The protein encoded by this gene is a T cell-derived cytokine that shares sequence similarity with IL17. This cytokine was reported to stimulate the release of TNF alpha (TNF) and IL1 beta (IL1B) from a monocytic cell line. Immunohistochemical analysis of several nerve tissues indicated that this cytokine is primarily localized to neuronal cell bodies. Alternative splicing results in multiple splice variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES

Human IL17B protein, His tag (active). GTX00134-pro

GTX00134-pro Functional Assay Image

ELISA detection of secreted IL-8 protein activation from A549 cells induced by GTX00134-pro Human IL17B protein (active) for 20hrs.
Recombinant protein concentrations: 250, and 500 ng/ml.

Human IL17B protein, His tag (active). GTX00134-pro

GTX00134-pro Image

SDS-PAGE analysis of GTX00134-pro Human IL17B protein (active).

Human IL17B protein, His tag (active). GTX00134-pro

GTX00134-pro Image

WB analysis of GTX00134-pro Human IL17B protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 219