APPLICATION
Application Note
IL2 (Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response. Recombinant human IL2 shares 56% AA sequence identity with mouse IL2, suggesting the exist of cross-species activity. Therefore, in order to detect the bioactivity of rhIL2, a cell proliferation assay has been conducted using CTLL-2 mouse cytotoxic T cells. Briefly, CTLL-2 cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well with or without the addition of various concentrations of IL2. After incubated for 48h, cells were observed by inverted microscope. Proliferation of CTLL-2 cells after incubation with IL2 for 48h observed by inverted microscope.
Observed MW
18 kDa.
PROPERTIES
Form
Lyophilized powder
Buffer
Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.6), 5% Trehalose.
Preservative
No preservative
Storage
For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.
Region/Sequence
N-terminal His-Tag; Ala21~Thr153 (NP_000577.2)
Expression System
HEK293 cells
Purity
> 95%
Endotoxin
< 1 EU/μg
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
interleukin 2 , IL-2 , TCGF , lymphokine
Background
The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX00092-pro Functional Assay Image
The dose effect curve of GTX00092-pro Human IL2 protein (active) on CTLL-2 cells. After incubation of Human IL2 protein (active) for48 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).
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GTX00092-pro Functional Assay Image
Cell proliferation effect of GTX00092-pro Human IL2 protein (active).
(A) Unstimulated CTLL-2 cells cultured in RPMI-1640 for 48hrs.
(B) CTLL-2 cells cultured in RPMI-1640, stimulated with 10 ng/ml IL2 for 48hrs.
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GTX00092-pro Image
WB analysis of GTX00092-pro Human IL2 protein (active).
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GTX00092-pro Image
SDS-PAGE analysis of GTX00092-pro Human IL2 protein (active).
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REFERENCE
There are currently no references for Human IL2 protein, His tag (active) (GTX00092-pro). Be the first to share your publications with this product.
REVIEW
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