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Human IL2 protein (active)

Human IL2 protein (active). GTX00265-pro
Human IL2 protein (active). GTX00265-pro
Human IL2 protein (active). GTX00265-pro

Cat No. GTX00265-pro

Application Functional Assay
Species Human
Package
10 μg ($239)
APPLICATION

Application Note

Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells. In the thymus, where T cells mature, it prevents autoimmune diseases by promoting the differentiation of certain immature T cells into regulatory T cells, which suppress other T cells that are otherwise primed to attack normal healthy cells in the body. IL-2 also promotes the differentiation of T cells into effector T cells and into memory T cells when the initial T cell is also stimulated by an antigen, thus helping the body fight off infections. To measure the effect of IL-2 on cell proliferation, CTLL-2 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well with 2% serum standard RPMI-1640 contain various concentrations of recombinant human IL-2. After incubated for 96h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of CTLL-2 cells after incubation with IL-2 for 96h observed by inverted microscope.

Observed MW

17 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala21~Thr153 (NP_000577.2)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 2 , IL-2 , TCGF , lymphokine

Background

The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human IL2 protein (active). GTX00265-pro

GTX00265-pro Functional Assay Image

Cell proliferation effect of GTX00265-pro Human IL2 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated CTLL-2 cells cultured in RPMI-1640 for 96hrs.
(B) CTLL-2 cells cultured in RPMI-1640, stimulated with 10 ng/ml IL-2 for 96hrs.

Human IL2 protein (active). GTX00265-pro

GTX00265-pro Image

SDS-PAGE analysis of GTX00265-pro Human IL2 protein (active).

Human IL2 protein (active). GTX00265-pro

GTX00265-pro Image

WB analysis of GTX00265-pro Human IL2 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 239