GeneTex
  • Country / Location Selection

United States (US)

Human IL22 protein (active)

Human IL22 protein (active). GTX00189-pro
Human IL22 protein (active). GTX00189-pro
Human IL22 protein (active). GTX00189-pro

Cat No. GTX00189-pro

Application Functional Assay
Species Human
Package
10 μg ($279)
APPLICATION

Application Note

Interleukin 22 (IL22) is a member of a group of cytokines called the IL-10 family or IL-10 superfamily, a class of potent mediators of cellular inflammatory responses. It shares use of IL-10R2 in cell signaling with other members of this family, IL-10, IL-26, IL-28A/B and IL-29. IL-22 is produced by activated NK and T cells and initiates innate immune responses against bacterial pathogens especially in epithelial cells such as respiratory and gut epithelial cells. IL-22 biological activity is initiated by binding to a cell-surface complex composed of IL-22R1 and IL-10R2 receptor chains and further regulated by interactions with a soluble binding protein. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10. Besides, Interleukin 10 Receptor Beta (IL10Rb) has been identified as an interactor of IL22, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL22 and recombinant human IL10Rb. Briefly, IL22 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to IL10Rb-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-IL22 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of IL22 and IL10Rb was in a dose dependent manner.

Observed MW

21 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Ala34~Ile179 (NP_065386.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 22,IL-21,IL-22,IL-D110,IL-TIF,ILTIF,TIFIL-23,TIFa,zcyto18

Background

This gene is a member of the IL10 family of cytokines that mediate cellular inflammatory responses. The encoded protein functions in antimicrobial defense at mucosal surfaces and in tissue repair. This protein also has pro-inflammatory properties and plays a role in in the pathogenesis of several intestinal diseases. [provided by RefSeq, Jul 2018]

Database

DATA IMAGES
Human IL22 protein (active). GTX00189-pro

GTX00189-pro Functional Assay Image

Functional ELISA analysis of GTX00189-pro Human IL22 protein (active) which can bind immobilized IL10Rb protein.

Human IL22 protein (active). GTX00189-pro

GTX00189-pro Image

WB analysis of GTX00189-pro Human IL22 protein (active).

Human IL22 protein (active). GTX00189-pro

GTX00189-pro Image

SDS-PAGE analysis of GTX00189-pro Human IL22 protein (active).

REFERENCE
There are currently no references for Human IL22 protein (active) (GTX00189-pro). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Human IL22 protein (active) (GTX00189-pro). Be the first to share your experience with this product.
Package List Price ($)
$ 279