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Human IL24 protein (active)

Human IL24 protein (active). GTX00138-pro
Human IL24 protein (active). GTX00138-pro
Human IL24 protein (active). GTX00138-pro
Human IL24 protein (active). GTX00138-pro

Cat No. GTX00138-pro

Application Functional Assay
Species Human
Package
10 μg ($239)
APPLICATION

Application Note

IL24 (interleukin 24) is a cytokine that belongs to IL10 family. This protein can induce apoptosis selectively in various cancer cells, including ECV304. Thus, inhibition of cell proliferation assay of IL24 was conducted using ECV-304 cells. Briefly,  ECV-304 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard RPMI-1640  prior to the addition of various concentrations of IL24. After incubated for 48h,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Inhibition of ECV-304 cells proliferation after incubation with IIL24 for 48h observed by inverted microscope. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with various concentrations of IL24 for 48h. The mean OD value of ECV-304 assessed by CCK-8 that IL24 significantly decreased cell viability of ECV-304 cells.

Observed MW

18 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gly51~Leu206 (NP_001172085.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 24 , C49A , FISP , IL10B , MDA7 , MOB5 , ST16

Background

This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Human IL24 protein (active). GTX00138-pro

GTX00138-pro Functional Assay Image

The inhibitory effect of GTX00138-pro Human IL24 protein (active) on the cell proliferation of ECV-304 cells. After incubation of Human IL24 protein (active) for 48 hrs, cell viability was measured by Cell Counting Kit-8 (CCK-8).

Human IL24 protein (active). GTX00138-pro

GTX00138-pro Functional Assay Image

Cell proliferation effect of GTX00138-pro Human IL24 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated ECV-304 cells cultured in serum-free RPMI-1640 for 48hrs.
(B) ECV-304 cells cultured in RPMI-1640, stimulated with 0.1 ng/ml IL24 for 48hrs.

Human IL24 protein (active). GTX00138-pro

GTX00138-pro Image

WB analysis of GTX00138-pro Human IL24 protein (active).

Human IL24 protein (active). GTX00138-pro

GTX00138-pro Image

SDS-PAGE analysis of GTX00138-pro Human IL24 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 239