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Human IL6 protein (active)

Human IL6 protein (active). GTX00094-pro
Human IL6 protein (active). GTX00094-pro
Human IL6 protein (active). GTX00094-pro

Cat No. GTX00094-pro

Application Functional Assay
Species Human
Package
10 μg ($259)
APPLICATION

Application Note

Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL-6 significantly increased the MMP-10 protein lever in the human lung cancer A549 cells through the JAK/STAT signaling pathway. Briefly, A549 cells were seeded into 6-well cell culture clusters and allowed to grow to 50-70% confluence, then different concentrations of IL-6 was added. After incubated for 24h, the protein levels of MMP-10 in the cell supernatant were determined by Western blot. MMP-10 protein lever significantly increased in A549 cells due to the stimulation of IL-6.

Observed MW

47 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His and GST-Tag; Pro29~Met212 (NP_000591.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

interleukin 6,BSF-2,BSF2,CDF,HGF,HSF,IFN-beta-2,IFNB2,IL-6

Background

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]

Database

Research Area

DATA IMAGES
Human IL6 protein (active). GTX00094-pro

GTX00094-pro Functional Assay Image

Activation of MMP10 protein expression on A549 cells induced by GTX00094-pro Human IL6 protein (active) for 24hrs.
Recombinant protein concentrations: 25, and 50 ng/ml.

Human IL6 protein (active). GTX00094-pro

GTX00094-pro Image

SDS-PAGE analysis of GTX00094-pro Human IL6 protein (active).

Human IL6 protein (active). GTX00094-pro

GTX00094-pro Image

WB analysis of GTX00094-pro Human IL6 protein (active).

REFERENCE
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REVIEW
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Package List Price ($)
$ 259