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Human JAK2 protein, His tag

Human JAK2 protein, His tag. GTX00152-pro
Human JAK2 protein, His tag. GTX00152-pro
Human JAK2 protein, His tag. GTX00152-pro

Cat. No. GTX00152-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($299)
See all JAK2 protein products

APPLICATION

Application Note

JAK2 (Tyrosine-protein kinase JAK2) is a tyrosine kinase involved in various processes such as cell growth, development, differentiation or histone modifications. JAK2 is considered to associate with some type I receptors, including EPOR (Erythropoietin receptor), therefore participates in cellular signal transduction. Thus a binding ELISA assay was conducted to detect the interaction of recombinant human JAK2 and recombinant human EPOR. Briefly, JAK2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl JAK2 were then transferred to EPOR-coated microtiter wells and incubated for 2h at 37ºC. Wells were washed with PBST and incubated for 1h with anti-JAK2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37ºC. Finally, add 50 μl stop solution to the wells and read at 450nm immediately. The binding activity of JAK2 and EPOR was in a dose dependent manner.

Observed MW

35 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Asn508~Ala800 (NP_001309123.1)

Expression System

E. coli

Purity

> 90%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Janus kinase 2 , JTK10 , THCYT3

Background

This gene product is a protein tyrosine kinase involved in a specific subset of cytokine receptor signaling pathways. It has been found to be constituitively associated with the prolactin receptor and is required for responses to gamma interferon. Mice that do not express an active protein for this gene exhibit embryonic lethality associated with the absence of definitive erythropoiesis. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human JAK2 protein, His tag. GTX00152-pro

GTX00152-pro Functional Assay Image

Functional ELISA analysis of GTX00152-pro Human JAK2 protein which can bind immobilized EPOR protein.

Human JAK2 protein, His tag. GTX00152-pro

GTX00152-pro Image

WB analysis of GTX00152-pro Human JAK2 protein.

Human JAK2 protein, His tag. GTX00152-pro

GTX00152-pro Image

SDS-PAGE analysis of GTX00152-pro Human JAK2 protein.

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 299