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Human MDH1 protein, His tag (active)

Human MDH1 protein, His tag (active). GTX67090-pro

Cat No. GTX67090-pro

Application

 Functional Assay

Species

 Human
Package
100 μg ($289)
See all MDH1 protein products

APPLICATION

Application Note

Specific activity is > 250 units/mg, and is defined as the amount of enzyme that cleaves 1.0 μmole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH 8.0 at 37ºC.

PROPERTIES

Form

Liquid

Buffer

20 mM Tris-HCl buffer (pH 8.0) with 10% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml

Region/Sequence

Full length protein, C-terminal His-Tag; Length: 342 a.a. Sequence: MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIATDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH

Expression System

E.coli

Purity

> 95% by SDS-PAGE.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

malate dehydrogenase 1 , HEL-S-32 , MDH-s , MDHA , MGC:1375 , MOR2

Background

This gene encodes an enzyme that catalyzes the NAD/NADH-dependent, reversible oxidation of malate to oxaloacetate in many metabolic pathways, including the citric acid cycle. Two main isozymes are known to exist in eukaryotic cells: one is found in the mitochondrial matrix and the other in the cytoplasm. This gene encodes the cytosolic isozyme, which plays a key role in the malate-aspartate shuttle that allows malate to pass through the mitochondrial membrane to be transformed into oxaloacetate for further cellular processes. Alternatively spliced transcript variants have been found for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Pseudogenes have been identified on chromosomes X and 6. [provided by RefSeq, Feb 2016]

Database

Research Area

DATA IMAGES

Human MDH1 protein, His tag (active). GTX67090-pro

GTX67090-pro Image

3 μg of GTX67090-pro Human MDH1 protein (active) by SDS-PAGE under reducing condition and visualized by coomassie blue stain

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REVIEW

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SDS
Glycerol.pdf
Package List Price ($)
$ 289