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Human MIP3 beta protein (active)

Human MIP3 beta protein (active). GTX00424-pro
Human MIP3 beta protein (active). GTX00424-pro
Human MIP3 beta protein (active). GTX00424-pro

Cat No. GTX00424-pro

Application Functional Assay
Species Human
Package
10 μg ($269)
APPLICATION

Application Note

Macrophage Inflammatory Protein 3 Beta (MIP3b) is a small cytokine belonging to the CC chemokine family that is also known as EBI1 ligand chemokine (ELC) and Chemokine C-C motif ligand 19 (CCL19). This chemokine elicits its effects on its target cells by binding to the chemokine receptor chemokine receptor CCR7. It attracts certain cells of the immune system, including dendritic cells and antigen-engaged B cells, CCR7 central-memory T-Cells. Thus, chemotaxis assay used 24-well microchemotaxis system was undertaken to detect the chemotactic effect of recombinant human MIP3b on the Jurkat cell line. Briefly, Jurkat cells were seeded into the upper chambers (150 μl cell suspension,10⁶ cells/ml in RPMI-1640 with FBS free) and MIP3b (0.01, 0.1, 1, 10, 100, and 1000 ng/ml diluted separately in serum free RPMI-1640) was added in lower chamber with a polycarbonate filter (8 μm pore size) used to separate the two compartments. After incubation at 37ºC with 5% CO₂ for 3h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×100) and the number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter). MIP3b is able to induce migration of Jurkat cells, and the migrated cells were counted at high magnification (×400). The optimum chemotaxis of recombinant human MIP3b occurs at 0.1-1 ng/ml.

Observed MW

14 kDa.
PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. Reconstitute with 20 mM Tris (pH8.0), 150 mM NaCl to a concentration of 0.1-1.0 mg/ml. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Gly22~Ser98 (NP_006265.1)

Expression System

E. coli

Purity

> 97%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

C-C motif chemokine ligand 19,CKb11,ELC,MIP-3b,MIP3B,SCYA19

Background

This antimicrobial gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene may play a role in normal lymphocyte recirculation and homing. It also plays an important role in trafficking of T cells in thymus, and in T cell and B cell migration to secondary lymphoid organs. It specifically binds to chemokine receptor CCR7. [provided by RefSeq, Sep 2014]

Database

DATA IMAGES
Human MIP3 beta protein (active). GTX00424-pro

GTX00424-pro Functional Assay Image

The chemotactic effect of GTX00424-pro Human MIP3 beta protein (active).
Upper: (A) Jurkat cells were seeded into the upper chambers and serum free RPMI-1640 without MIP3b was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
(B) Jurkat cells were seeded into the upper chambers and serum free RPMI-1640 with 0.1 ng/ml MIP3b was added in lower chamber, then cells in lower chamber were observed at low magnification (×100) after incubation for 3hrs.
Lower: The number of migrated cells were counted at high magnification (×400) randomly (five fields for each filter).

Human MIP3 beta protein (active). GTX00424-pro

GTX00424-pro Image

WB analysis of GTX00424-pro Human MIP3 beta protein (active).

Human MIP3 beta protein (active). GTX00424-pro

GTX00424-pro Image

SDS-PAGE analysis of GTX00424-pro Human MIP3 beta protein (active).

REFERENCE
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Package List Price ($)
$ 269