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Human MMP9 protein, His tag (Active)

Human MMP9 protein, His tag (Active). GTX02551-pro
Human MMP9 protein, His tag (Active). GTX02551-pro
Human MMP9 protein, His tag (Active). GTX02551-pro

Cat. No. GTX02551-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($359)
See all MMP9 protein products

APPLICATION

Application Note

MMP9 is a zinc-dependent enzymes capable of cleaving components of the extracellular matrix, which belongs to the matrix metalloproteinase (MMP) family. It is a gelatinase A, 92kDa type IV collagenase which can hydrolyze gelatin under certain conditions. Gelatin zymography is mainly used for the detection of the gelatinases, MMP-2 and MMP-9, and it is extremely sensitive because levels of 10pg of MMP-2 can already be detected. Briefly, various concentrations of recombinant human MMP9 (1000ng, 500ng, 100ng, 10ng) were denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate- polyacrylamide gel (SDS-PAGE; 10% gels) containing gelatin (1 mg/ml) with nonreducing conditions. After renaturation, incubation and CCB-stained, active MMP2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel. In this experiment we use heat-denatured MMP9 protein as negative control, and blood sample as positive control.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris (pH8.0) and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris (pH8.0), 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tagged; Gly213-Ala399

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1.0 EU per μg (determined by the LAL method)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

matrix metallopeptidase 9 , CLG4B , GELB , MANDP2 , MMP-9

Background

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Human MMP9 protein, His tag (Active). GTX02551-pro

GTX02551-pro Image

WB analysis of GTX02551-pro Human MMP9 protein.

Human MMP9 protein, His tag (Active). GTX02551-pro

GTX02551-pro Image

SDS-PAGE of GTX02551-pro Human MMP9 protein, His tag (Active).

Human MMP9 protein, His tag (Active). GTX02551-pro

GTX02551-pro Functional Assay Image

Gelatin hydrolysis by various concentrations of GTX02551-pro Human MMP9 protein, His tag (Active).
Positive control : blood sample
Negative control : heat-denatured MMP9 protein

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Package List Price ($)
$ 359