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Human Progranulins protein (active)

Human Progranulins protein (active). GTX65641-pro
Human Progranulins protein (active). GTX65641-pro

Cat No. GTX65641-pro

Application Functional Assay
Species Human
10 μg ($289)

Application Note

Activates phospho-ERK1/2 in neuronal mouse P19 cells. Regulates food intake and body weight.


Lyophilized powder


Lyophilized from 0.2μm-filtered PBS. Reconstitute with 100 μl ddH₂O.


Store at -20ºC or below. After reconstitution, keep as concentrated solution. Aliquot and avoid freeze-thaw cycles.


Signal peptide and human progranulin (a.a. 1-593); reflects the native with no additional amino acid.

Expression System

HEK293 cells


≥ 98% by SDS-PAGE.


< 0.01 EU/μg




For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.


granulin precursor , CLN11 , GEP , GP88 , PCDGF , PEPI , PGRN


Research Area

Human Progranulins protein (active). GTX65641-pro

GTX65641-pro Image

The effects on phospho-ERK1/2 and ERK1/2 by GTX65641-pro Progranulin protein in neuronal differentiated mouse P19 cells. Undifferentiated mouse P19 cells were induced to differentiated in 1μM retinoic acid (RA) in α-minimum essential medium (αMEM) containing 10% heat-treated fetal bovine serum on bacterial grade plates for 3~4 days to allow aggregates to form (generation of embryonic bodies). The aggregates were then plated on tissue culture grade plates in the absence of RA for 3~4days. To examine the induction of signal of phospho-ERK1/2 and ERK1/2, reactions were carried out at 37°C over 0, 5, 10, 30, 60, 120mins, respectively by adding the recombinant protein (500ng/ml) to the neuronal differentiated mouse P19 cells, which were maintained with serum starvation for 24hrs. Treatment with Progranulin protein (GTX65641-pro) was performed in lanes 1, 2, 3, 4, 5, and 6 over 0, 5, 10, 30, 60, 120mins, respectively. GAPDH was used as loading control for western blotting.

Human Progranulins protein (active). GTX65641-pro

GTX65641-pro Image

Deglycosylation of GTX65641-pro Progranulin (human) protein. To examine the deglycosylation of human Progranulin, 1 μg of human progranulin is denatured with 1X glycoprotein denaturing buffer at 100°C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two fold dilutions of PNGase F are added and the reaction mix is incubated for 1 hour at 37°C. Separation of reaction products is visualized by immunoblotting using anti-Progranulin (human) antibody.

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Package List Price ($)
$ 289