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Human Prolactin protein, His tag (active)

Human Prolactin protein, His tag (active). GTX00069-pro
Human Prolactin protein, His tag (active). GTX00069-pro
Human Prolactin protein, His tag (active). GTX00069-pro
Human Prolactin protein, His tag (active). GTX00069-pro

Cat. No. GTX00069-pro

Application

 Functional Assay

Species

 Human
Package
10 μg ($349)
See all Prolactin protein products

APPLICATION

Application Note

PRL (prolactin), also known as luteotropin, is a hormone secreted from the pituitary gland and is best known for its role in enabling mammals to produce milk. PRL plays an essential role in metabolism, regulation of the immune system. According to reports, PRL also stimulates proliferation of certain cells, including MCF-7. Thus, proliferation assay of PRL was conducted using MCF-7 cells. Briefly,  MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM   prior to the addition of various concentrations of PRL. After incubated for 72h,   cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Cell proliferation of MCF-7 cells after stimulated with PRL. And PRL significantly promoted cell proliferation of MCF-7 cells. The ED₅₀ for this effect is typically 3. 709-8. 973 ng/ml

Observed MW

24/28 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Leu29~Cys227 (NP_000939.1)

Expression System

HEK293 cells

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

prolactin , GHA1

Background

This gene encodes the anterior pituitary hormone prolactin. This secreted hormone is a growth regulator for many tissues, including cells of the immune system. It may also play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Human Prolactin protein, His tag (active). GTX00069-pro

GTX00069-pro Functional Assay Image

Cell proliferation effect of GTX00069-pro Human Prolactin protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated MCF-7 cells cultured in DMEM for 72hrs.
(B) MCF-7 cells cultured in DMEM, stimulated with 1 ng/ml PRL for 72hrs.

Human Prolactin protein, His tag (active). GTX00069-pro

GTX00069-pro Functional Assay Image

The dose effect curve of GTX00069-pro Human Prolactin protein (active) on MCF-7 cells. After incubation of Human Prolactin protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Human Prolactin protein, His tag (active). GTX00069-pro

GTX00069-pro Image

WB analysis of GTX00069-pro Human Prolactin protein (active).

Human Prolactin protein, His tag (active). GTX00069-pro

GTX00069-pro Image

SDS-PAGE analysis of GTX00069-pro Human Prolactin protein (active).

REFERENCE

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REVIEW

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SDS
Proclin.pdf
Package List Price ($)
$ 349