Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures such as those in tau fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to tau aggregation) when tau preformed fibrils (GTX17674-pro) are combined with tau monomers (GTX17672-pro). The preformed fibrils seed the formation of new fibrils from a pool of monomers. Thioflavin T ex = 450 nm, em = 485 nm.

SDS-PAGE of ~67 kDa Human Tau441 (2N4R) protein, mutant P301S (preformed fibrils (PFFs)) (GTX17674-pro).
Lane 1: MW Ladder. Lane 2: Human Tau441 (2N4R) protein, mutant P301S (fibrils)(GTX17674-pro).