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Human Transferrin protein, His tag (active)

Human Transferrin protein, His tag (active). GTX01373-pro
Human Transferrin protein, His tag (active). GTX01373-pro

Cat No. GTX01373-pro

Application

Functional Assay

Species

Human
Package
100 μg ($329),
50 μg ($249)

APPLICATION

Application Note

1. Measured by its binding ability in a functional ELISA. Immobilized human CD71 protein at 10 μg/ml (100 μl/well) can bind human Transferrin protein. The EC₅₀ of human Transferrin protein is 5.6 ng/ml.
2. Measured in a serum-free cell proliferation assay using MCF-7 cells. The ED₅₀ for this effect is typically 0.01-0.04 μg/ml.

Observed MW

74 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Batch dependent (please contact us for details)

Storage

Store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

C-terminal poly His tagged; Met1-Pro698 of Human Transferrin protein (NP_001054.1)

Expression System

HEK293 cells

Purity

> 95% by SDS-PAGE.

Endotoxin

< 1.0 EU/μg (determined by LAL method)

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

HEL-S-71p,PRO1557,PRO2086,TFQTL1

Background

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Human Transferrin protein, His tag (active). GTX01373-pro

GTX01373-pro Functional Assay Image

The protein activity of GTX01373-pro Human Transferrin protein was measured in a cell proliferation assay using MCF-7 cells.
ED₅₀ : 0.01-0.04 μg/ml

Human Transferrin protein, His tag (active). GTX01373-pro

GTX01373-pro Image

SDS-PAGE of 5 μg GTX01373-pro Human Transferrin protein, His tag (active).

REFERENCE

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Package List Price ($)
$ 329
$ 249