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Human alpha Synuclein protein (active, Pre-Formed Fibrils)

Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro
Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro
Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro
Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro
Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro
Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro
Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro

Cat No. GTX17669-pro

Application

Functional Assay

Species

Human
Package
200 μg ($509),
100 μg ($369)
PRODUCT

Summary

Active Human Recombinant Alpha Synuclein Pre-formed Fibrils (Type 1)

APPLICATION

Application Note

Endogenous alpha-synuclein phosphorylation. 100 μM alpha synuclein protein monomer (GTX17668-pro) seeded with 10 nM alpha synuclein protein PFF (GTX17669-pro) in 25 μM Thioflavin T (PBS pH 7.4, 100 μl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37ºC with shaking at 600 rpm. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Region/Sequence

Full-length without tagged; MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA

Expression System

E. coli

Purification

Purified by ion-exchange chromatography

Purity

> 95% by SDS-PAGE

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

synuclein alpha , synuclein alpha , NACP , PARK1 , PARK4 , PD1

Database

  • Gene ID: 6622 SNCA

Research Area

DATA IMAGES

Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro

GTX17669-pro Functional Assay Image

Primary rat hippocampal neurons show lewy body inclusion formation when treated with active Alpha Synuclein Protein Preformed Fibrils (GTX17669-pro) at 4 μg/ml (D-F), but not when treated with control Alpha Synuclein Protein Preformed Fibrils (GTX17667-pro) at 4 μg/ml (A-C).
Tissue: Primary hippocampal neurons. Species: Sprague-Dawley rat. Fixation: 4% formaldehyde from PFA. Primary Antibody: Mouse anti-pSer129 Antibody at 1:1000 24 hours at 4ºC. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:700 for 1 hours at RT. Counterstain: Hoechst (blue) nuclear stain at 1:4000 for 1 hour at RT. Localization: Lewy body inclusions. Magnification: 20x.

Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro

GTX17669-pro Functional Assay Image

Immunohistochemistry analysis of rat brain injected with active human alpha synuclein PFFs (GTX17669-pro). Species: Female Sprague-Dawley Rat. Rat was injected with 2μL active human alpha synuclein PFFs (GTX17669-pro) in each of 2 injection sites: AP+1.6, ML+2.4, DV-4.2 from skull; and AP-1.4, ML+0.2, DV-2.8 from skull. 30-days post-injection. Fixation: Saline perfusion followed by 4% PFA fixation for 48 hrs. Secondary Antibody: Biotin-SP Donkey Anti-Rabbit IgG (H+L) at 1:500 for 2 hours in cold room with shaking. ABC signal amplification, DAB staining. Magnification: 20X. Alpha synuclein pathology is seen in the striatum close to an injection site.

Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro

GTX17669-pro Image

Active alpha synuclein preformed fibrils (GTX17669-pro) seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers (GTX17668-pro). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift, and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of active alpha synuclein preformed fibrils (GTX17669-pro) is combined with 100 μM of alpha synuclein monomer (GTX17668-pro), as compared to active alpha synuclein preformed fibrils (GTX17669-pro) alone and alpha synuclein monomer (GTX17668-pro) alone. Thioflavin T ex = 450 nm, em = 485 nm.

Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro

GTX17669-pro Image

TEM of active human alpha synuclein preformed fibrils (GTX17669-pro). Fibrils were sonicated and treated with uranyl acetate. Top: 50kx magnification. Bottom: 100kx magnification.

Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro

GTX17669-pro Image

TEM of active human alpha synuclein preformed fibrils (GTX17669-pro) (top) and control human alpha synuclein preformed fibrils (GTX17667-pro) (bottom). Fibrils were sonicated and treated with uranyl acetate. The active fibrils are shorter than the control fibrils.

Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro

GTX17669-pro Image

TEM of active human alpha synuclein preformed fibrils (GTX17669-pro) (top) and control human alpha synuclein preformed fibrils (GTX17667-pro) (bottom). Fibrils were sonicated and treated with uranyl acetate. The active fibrils are shorter than the control fibrils.

Human alpha Synuclein protein (active, Pre-Formed Fibrils). GTX17669-pro

GTX17669-pro Image

SDS-PAGE of ~14 kDa active Human Recombinant Alpha Synuclein Protein Preformed Fibrils (GTX17669-pro). Lane 1: Molecular Weight Ladder (MW). Lane 2: active Alpha Synuclein Protein Preformed Fibrils (GTX17669-pro).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 509
$ 369