Active alpha synuclein preformed fibrils (GTX17669-pro) seed the formation of new alpha synuclein fibrils from the pool of alpha synuclein monomers (GTX17668-pro). Thioflavin T is a fluorescent dye that binds to beta sheet-rich structures, such as those in alpha synuclein fibrils. Upon binding, the emission spectrum of the dye experiences a red-shift and increased fluorescence intensity. Thioflavin T emission curves show increased fluorescence (correlated to alpha synuclein protein aggregation) over time when 10 nM of active alpha synuclein preformed fibrils (GTX17669-pro) is combined with 100 μM of alpha synuclein monomer (GTX17668-pro), as compared to when 10 nM of control alpha synuclein preformed fibrils (GTX17667-pro) is combined with 100 μM of alpha synuclein monomer (GTX17668-pro) or 100 μM of alpha Synuclein monomer (GTX17666-pro). Thioflavin T ex = 450 nm, em = 485 nm.

SDS-PAGE of ~14 kDa Human alpha Synuclein protein (active, monomer) (GTX17666-pro).
Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (2.5 μg). Lane 3: BSA (5 μg). Lane 4: Alpha Synuclein Protein Monomer (2.5 μg) (GTX17666-pro). Lane 5: Alpha Synuclein Protein Monomer (5 μg) (GTX17666-pro).