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Huntingtin antibody

Anti-Huntingtin antibody used in Western Blot (WB). GTX132433
Anti-Huntingtin antibody used in Western Blot (WB). GTX132433
Anti-Huntingtin antibody used in Western Blot (WB). GTX132433
Anti-Huntingtin antibody used in Western Blot (WB). GTX132433
Anti-Huntingtin antibody used in IHC (Paraffin sections) (IHC-P). GTX132433
Anti-Huntingtin antibody used in Western Blot (WB). GTX132433

Cat. No. GTX132433

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

348 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Zebrafish, Sheep, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Huntingtin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886646

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

huntingtin , HD , IT15 , LOMARS

Cellular Localization

Cytoplasm , Nucleus

Background

Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Huntingtin antibody used in Western Blot (WB). GTX132433

GTX132433 WB Image

Wild-type (WT) and HTT knockout (KO) 293T cell extracts (10 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (GTX132433) diluted at 1:1000. The HRP-conjated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Huntingtin antibody used in Western Blot (WB). GTX132433

GTX132433 WB Image

Non-transfected (–) and transfected (+) U87-MG whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (GTX132433) diluted at 1:1000.

Anti-Huntingtin antibody used in Western Blot (WB). GTX132433

GTX132433 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (GTX132433) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Huntingtin antibody used in Western Blot (WB). GTX132433

GTX132433 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (GTX132433) diluted at 1:1000.

Anti-Huntingtin antibody used in IHC (Paraffin sections) (IHC-P). GTX132433

GTX132433 IHC-P Image

Huntingtin antibody detects Huntingtin protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Huntingtin antibody (GTX132433) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Huntingtin antibody used in Western Blot (WB). GTX132433

GTX132433 WB Image

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (GTX132433) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for Huntingtin antibody (GTX132433). Be the first to share your publications with this product.

REVIEW

Huntingtin antibody Cat. No. GTX132433

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 28-Jun-2024
Score :
Application Tested : IP
Sample Species : Hu
Sample : DMS 53 cells
Lane Description :
SM=4% starting material
UB=4% unbound fraction
Figure Note :
Antibody-beads conjugates were prepared by adding 2 μg to 500 μl of IP Lysis Buffer in a microcentrifuge tube, together with with 30 μl of Dynabeads protein A- (for rabbit antibodies) or protein G- (for mouse and rat antibodies) beads.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Date : Anonymous submitted on 28-Jun-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : DMS 53 WT and HTT KO cells
Amount used : 30μg
Primary Antibody : 1:500, 4°C, 16Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169