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Huntingtin antibody

Anti-Huntingtin antibody used in Western Blot (WB). GTX132433
Anti-Huntingtin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132433
Anti-Huntingtin antibody used in IHC (Paraffin sections) (IHC-P). GTX132433
Anti-Huntingtin antibody used in Western Blot (WB). GTX132433

Cat No. GTX132433

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

348 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Sheep, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Huntingtin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Huntingtin,Hd,It15,Lomars,Htt

Cellular Localization

Cytoplasm , Nucleus

Background

Huntingtin is a disease gene linked to Huntington's disease, a neurodegenerative disorder characterized by loss of striatal neurons. This is thought to be caused by an expanded, unstable trinucleotide repeat in the huntingtin gene, which translates as a polyglutamine repeat in the protein product. A fairly broad range in the number of trinucleotide repeats has been identified in normal controls, and repeat numbers in excess of 40 have been described as pathological. The huntingtin locus is large, spanning 180 kb and consisting of 67 exons. The huntingtin gene is widely expressed and is required for normal development. It is expressed as 2 alternatively polyadenylated forms displaying different relative abundance in various fetal and adult tissues. The larger transcript is approximately 13.7 kb and is expressed predominantly in adult and fetal brain whereas the smaller transcript of approximately 10.3 kb is more widely expressed. The genetic defect leading to Huntington's disease may not necessarily eliminate transcription, but may confer a new property on the mRNA or alter the function of the protein. One candidate is the huntingtin-associated protein-1, highly expressed in brain, which has increased affinity for huntingtin protein with expanded polyglutamine repeats. This gene contains an upstream open reading frame in the 5' UTR that inhibits expression of the huntingtin gene product through translational repression. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Anti-Huntingtin antibody used in Western Blot (WB). GTX132433

GTX132433 WB Image

Non-transfected (–) and transfected (+) U87-MG whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (GTX132433) diluted at 1:1000.

Anti-Huntingtin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132433

GTX132433 ICC/IF Image

Huntingtin antibody detects Huntingtin protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Huntingtin protein stained by Huntingtin antibody (GTX132433) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-Huntingtin antibody used in IHC (Paraffin sections) (IHC-P). GTX132433

GTX132433 IHC-P Image

Huntingtin antibody detects Huntingtin protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Huntingtin antibody (GTX132433) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Huntingtin antibody used in Western Blot (WB). GTX132433

GTX132433 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Huntingtin antibody (GTX132433) diluted at 1:1000.

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169