Wild-type (WT) and IDE knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT143] (GTX633694) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT143] (GTX633694) diluted at 1:1000.

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IDE antibody [GT143] (GTX633694) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

IDE antibody [GT143] detects IDE protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
IDE antibody [GT143] (GTX633694) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

IDE antibody [GT143] detects IDE protein at cytoplasm in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
IDE antibody [GT143] (GTX633694) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min