APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , HepG2 , HeLa , Huh-7 , Neuro2A , C8D30 , NIH-3T3 , Raw 264.7 , C2Cl2 , DDDDk-tagged IDH1-transfected 293T
Predict Reactivity
Zebrafish, Sheep, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.69 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human IDH1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1950540
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
isocitrate dehydrogenase (NADP(+)) 1, cytosolic , HEL-216 , HEL-S-26 , IDCD , IDH , IDP , IDPC , PICD
Cellular Localization
Cytoplasm , Peroxisome
Background
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX105179 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody (GTX105179) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105179 WB Image
Wild-type (WT) andIDH1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody (GTX105179) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105179 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with IDH1 antibody (GTX105179) diluted at 1:1000 and competitor's antibody (CST#8137) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX105179 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IDH1(GTX105179) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX105179 IP Image
IDH-1 antibody immunoprecipitates IDH-1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of IDH-1 protein by 2.5 μg of IDH-1 antibody (GTX105179) 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody (GTX105179) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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GTX105179 WB Image
IDH1 antibody detects IDH1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell extract
B. 30 μg C8D30 whole cell extract
C. 30 μg NIH-3T3 whole cell extract
D. 30 μg Raw 264.7 whole cell extract
E. 30 μg C2Cl2 whole cell extract
10 % SDS-PAGE
IDH1 antibody (GTX105179) dilution: 1:1000
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GTX105179 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody (GTX105179) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105179 WB Image
Wild-type (WT) and IDH1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody (GTX105179) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105179 IHC-P Image
IDH1 antibody detects IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.IDH1 stained by IDH1 antibody (GTX105179) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX105179 IHC-P Image
IDH1 antibody detects IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.IDH1 stained by IDH1 antibody (GTX105179) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX105179 IHC-P Image
IDH1 antibody detects IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.IDH1 stained by IDH1 antibody (GTX105179) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX105179 IHC-P Image
IDH1 antibody detects IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.IDH1 stained by IDH1 antibody (GTX105179) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX105179 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody (GTX105179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105179 IHC-P Image
IDH1 antibody detects IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.IDH1 stained by IDH1 antibody (GTX105179) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX105179 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody (GTX105179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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REFERENCE
REVIEW
IDH1 antibody
Cat No. GTX105179
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Rating
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( Average 5 based on 1 users reviews)
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Application
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Western Blot(WB)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 20-Aug-2019
Application Tested :
WB
Sample Species :
Ms
Sample :
C57BL/6J mice kidney
Amount used :
20 μg
Blocking :
0.25% PVP-40 in TBST, 25°C, 1Hr
Primary Antibody :
1:2000, 4°C, 18Hr
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