APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
A431 , HepG2 , HeLa , Huh-7 , Neuro2A , C8D30 , NIH-3T3 , Raw 264.7 , C2Cl2 , DDDDk-tagged IDH1-transfected 293T
Predict Reactivity
Zebrafish, Sheep, Bovine, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human IDH1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
Isocitrate Dehydrogenase (Nadp(+)) 1, Cytosolic,Hel-216,Hel-S-26,Idcd,Idh,Idp,Idpc,Picd,Idh1
Cellular Localization
Cytoplasm , Peroxisome
Background
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX105179 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody (GTX105179) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX105179 WB Image
Wild-type (WT) and IDH1 knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody (GTX105179) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
CRISPR knockout cell lines generated by 
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GTX105179 WB Image
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with IDH1 antibody (GTX105179) diluted at 1:1000 and competitor's antibody (CST#8137) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX105179 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IDH1(GTX105179) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX105179 IP Image
IDH-1 antibody immunoprecipitates IDH-1 protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of IDH-1 protein by 2.5 μg of IDH-1 antibody (GTX105179) 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody (GTX105179) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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GTX105179 IHC-P Image
IDH1 antibody detects IDH1 protein at cytoplasm in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
IDH1 antibody (GTX105179) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX105179 WB Image
IDH1 antibody detects IDH1 protein by Western blot analysis.
A. 30 μg Neuro2A whole cell extract
B. 30 μg C8D30 whole cell extract
C. 30 μg NIH-3T3 whole cell extract
D. 30 μg Raw 264.7 whole cell extract
E. 30 μg C2Cl2 whole cell extract
10 % SDS-PAGE
IDH1 antibody (GTX105179) dilution: 1:1000
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GTX105179 IHC-P Image
IDH1 antibody detects IDH1 protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
IDH1 antibody (GTX105179) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX105179 WB Image
IDH1 antibody detects IDH1 protein by Western blot analysis.
A. 30 μg A431 whole cell extract
B. 30 μg HepG2 whole cell extract
10 % SDS-PAGE
IDH1 antibody (GTX105179) dilution: 1:1000
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GTX105179 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody (GTX105179) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
