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IDH3A antibody

Anti-IDH3A antibody used in Western Blot (WB). GTX104073
Anti-IDH3A antibody used in IHC (Paraffin sections) (IHC-P). GTX104073
Anti-IDH3A antibody used in Western Blot (WB). GTX104073
Anti-IDH3A antibody used in IHC (Paraffin sections) (IHC-P). GTX104073
Anti-IDH3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104073
Anti-IDH3A antibody used in Western Blot (WB). GTX104073
Anti-IDH3A antibody used in Western Blot (WB). GTX104073

Cat. No. GTX104073

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all IDH3A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human IDH3A. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950542

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

isocitrate dehydrogenase 3 (NAD(+)) alpha

Cellular Localization

Mitochondrion

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IDH3A antibody used in Western Blot (WB). GTX104073

GTX104073 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (GTX104073) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IDH3A antibody used in IHC (Paraffin sections) (IHC-P). GTX104073

GTX104073 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using IDH3A antibody (GTX104073) at 1:300 dilution.

Anti-IDH3A antibody used in Western Blot (WB). GTX104073

GTX104073 WB Image

Sample (30 μg of whole cell lysate)
A: whole zebrafish
B: zebrafish eye
C: zebrafis heart
10% SDS PAGE
GTX104073 diluted at 1:1000

Anti-IDH3A antibody used in IHC (Paraffin sections) (IHC-P). GTX104073

GTX104073 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human lung SCC, using IDH3A(GTX104073) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-IDH3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104073

GTX104073 ICC/IF Image

IDH3A antibody detects IDH3A protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: IDH3A stained by IDH3A antibody (GTX104073) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-IDH3A antibody used in Western Blot (WB). GTX104073

GTX104073 WB Image

IDH3A antibody detects IDH3A protein by western blot analysis.
A. 50 μg rat brain lysate/extract
10% SDS-PAGE
IDH3A antibody (GTX104073) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IDH3A antibody used in Western Blot (WB). GTX104073

GTX104073 WB Image

Sample (20 μg of whole cell lysate)
A: mouse brain
10% SDS PAGE
GTX104073 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Reynolds MS et al. Am J Physiol Endocrinol Metab 2016; 311 (1) : E186-201 β-cell deletion of nr4a1 and nr4a3 nuclear receptors impedes mitochondrial respiration and * secretion.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169