Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (GTX104073) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (GTX104073) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (GTX104073) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (GTX104073) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH3A antibody (GTX104073) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IDH3A antibody detects IDH3A protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: IDH3A stained by IDH3A antibody (GTX104073) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Immunohistochemical analysis of paraffin-embedded human lung SCC, using IDH3A(GTX104073) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using IDH3A antibody (GTX104073) at 1:300 dilution.