Untreated (–) and treated (+) A549 whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IFIT3 antibody [HL2469] (GTX638818) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

IFIT3 antibody [HL2469] detects IFIT3 protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded human glioblastoma.
IFIT3 stained by IFIT3 antibody [HL2469] (GTX638818) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min