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IGF1R alpha antibody [AMY10-105.8 (1H7)] (Low endotoxin, azide free)

Anti-IGF1R alpha antibody [AMY10-105.8 (1H7)] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX42246

Cat. No. GTX42246

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AMY10-105.8 (1H7)

Isotype

 IgG1

Application

 WB, IHC-P, FACS, Neutralizing/Inhibition

Reactivity

 Human
Package
100 μg ($399)
See all IGF1R products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1/10-1/40
FACS 1/10
Neutralizing/Inhibition Assay dependent

Note :

IHC-P
This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.

FACS
Use 10μl of the suggested working dilution to label 106 cells in 100μl.

Neutralizing/Inhibition
Clone 1H7 is reported to partially block binding of IGF-I and IGF-II to CD221 (Li et al.1993).
Not tested in other applications.

Calculated MW

155 kDa. ( Note )

Product Note

This antibody recognizes an epitope in the alpha subunits of IGF1R.Clone 1H7 is reported to partially block binding of IGF-I and IGF-II to IGF1 Receptor (Li et al.1993).

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.0 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human placental IGF-I receptor.

Purification

Protein G purified
From tissue culture supernatant

Endotoxin

< 0.01 EU/μg (determined by the LAL assay)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

insulin like growth factor 1 receptor , CD221 , IGFIR , IGFR , JTK13

Cellular Localization

Cell membrane

Background

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, May 2014]

Database

Research Area

DATA IMAGES

Anti-IGF1R alpha antibody [AMY10-105.8 (1H7)] (Low endotoxin, azide free) used in Flow cytometry (FACS). GTX42246

GTX42246 FACS Image

FACS analysis of human peripheral blood granulocytes using GTX42246 IGF1R alpha antibody [AMY10-105.8 (1H7)] (Low endotoxin, azide free).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations